PURPOSE The purpose of this study was to determine whether transient hypoxia had an effect on transforming growth factor beta1 (TGFbeta1)-induced rabbit corneal keratocyte myofibroblast transformation. METHODS Primary isolated rabbit corneal keratocytes were cultured in a serum-free medium. The effect of transient hypoxia treatment (1% oxygen, 4 h/day) on TGFbeta1 (5 ng/ml)-induced alpha-smoo...

PURPOSE TGFbeta is the major mediator to induce myofibroblast differentiation in the corneal wound-healing process. Elevated cAMP can reduce TGFbeta-induced fibrosis in other tissues. This study was conducted to determine whether elevated cAMP can inhibit TGFbeta1-induced rabbit corneal keratocyte-myofibroblast transformation. METHODS Primary isolated rabbit corneal keratocytes were cultured ...

i?&bQ!k A total of 200 normal human corneas (age: 6 months 92 years) were collected among bodies intended for autopsy. Pathological comeal buttons were obtained from patients undergoing penetrating keratoplasty for keratoconus, Fuchs’ end&&xl dyamphy, postcataract endothelial dystrophy, and others. The keratocyte density was assessed using a quantitative meawem eat of the stmmal DNA-content. Th...

i?&bQ!k A total of 200 normal human corneas (age: 6 months 92 years) were collected among bodies intended for autopsy. Pathological comeal buttons were obtained from patients undergoing penetrating keratoplasty for keratoconus, Fuchs’ end&&xl dyamphy, postcataract endothelial dystrophy, and others. The keratocyte density was assessed using a quantitative meawem eat of the stmmal DNA-content. Th...