The metabolism of inositol 1,4,5-trisphosphate [Ins(1,4,5)P3] was examined in yeast cells and germ tubes of Candida albicans. Methods have been developed for analysis of the two key metabolic enzymes, Ins(1,4,5)P3, kinase and phosphatase. ATP-dependent Ins(1,4,5)P3 kinase activity was detected predominantly in the soluble fraction of cell extracts and exhibited a Km of approximately 9 microM. T...

PUtOXY5MAL NOCTURNAL HEMosLoBrxu1tI.s (P.N.H.) is a chronsic hemolytic disease which affects all the formed blood elements. Once the disease is established these elemensts, includinig their boise marrow precursors, becomsse liable to excessive destructions. We do not yet knsow what causes the blood elements ins P.N.H. to be abmsornnahly labile, though the mechanism insvolved ins their destrunct...

myo-Inositol (Ins) and its polyphosphoinositide derivatives that are important in membrane signaling have long been held to play a special role in brain metabolism. As polyphosphoinositides turn over rapidly and are exceptionally abundant in nervous tissue, high Ins levels in the range of 2-15 mm that have been observed in brain may be necessary to maintain the rates of phosphoinositide synthes...

Zot is an agile and easily extendible bounded model checker, which can be downloaded at http://home.dei.polimi.it/pradella/. The tool supports different logic languages through a multi-layered approach: its core uses PLTL, and on top of it a decidable predicative fragment of TRIO is defined. An interesting feature of Zot is its ability to support different encodings of temporal logic as SAT pro...

The interaction of the only reported plant inositol trisphosphate receptor with different myo-inositol trisphosphates (InsP3 species), namely Ins(1,4,5)P3, Ins(1,3,4)P3, Ins(1,5,6)P3, and Ins(2,4,5)P3, were studied to assess the extent of Ca2+ mobilization from microsomes/vacuoles as well as liposomes in vitro. Ins(1,4,5)P3 and Ins(2,4,5)P3 bind with the receptor with comparable affinities, as ...

Inositol (1,4,5)-trisphosphate [Ins(1,4,5)P(3)] originating in the vascular smooth-muscle cells (VSMCs) has been shown to modulate the Ca(2+) stores in endothelial cells (ECs). However, the reverse is not found, suggesting that Ins(1,4,5)P(3) movement might be unidirectional across gap junctions at the myoendothelial junction (MEJ), or that distribution of the Ins(1,4,5)P(3) receptor [Ins(1,4,5...

ARMSim# is a simulator for the ARM architecture intended for use in both teaching and research. The simulator provides some standard features found in debuggers such as breakpoints, singlestep execution and watch-points. Its innovative features include support for software plug-ins which can implement external devices and new instructions. Such plug-ins can be used to prepare students for worki...

Code Bubbles is an attempt to redefine the user interface for an integrated programming environment. As it represents a whole new user interface, implementing it as a plug-in is inherently difficult. We get around this difficulty by combining two different plug-in architectures, a standard one based on registrations and callbacks, and a message-based one that puts the plug-in at arm’s length an...

An assay is described which allows the determination of the mass of any individual inositol phosphate (InsP) isomer by combining a popular h.p.l.c. separation method with simple desalting, dephosphorylation and final measurement of Ins liberated using an inositol dehydrogenase-NADH-linked bioluminescence reaction. The limit of sensitivity of this assay is about 1 pmol of Ins. routinely 5 pmol. ...