Background: Fenugreek (Trigonella foenum-graecum L.) is an important medicinal plant that is a source of diosgenin, trigonelline and other valuable components. Fenugreek seeds is used for its pharmaceutical and nutraceutical properties. Objective: The aim of present study was to investigate the genetic diversity within and between different populations of Trigonella foenum-graecum using DNA ma...

Inter-simple sequence repeats markers were used to determinate the genetic variability of Fasciola hepatica populations recovered from sheep and cattle from Spain (Sp1, Sp2, Sp3 and Sp4), UK (Eng), Ireland (Ir) and Mexico (Mex). Twenty five primers were tested but only five produced 39 reproducible bands, being 71.79% polymorphic bands. This percentage ranged from 10.26% in Sp4 to 48.72% in Sp1...

The genus Labisia (Myrsinaceae) is a popular medicinal plant in Malaysia. We examined the genetic relationship among three varieties of L. pumila var. pumila, L. pumila var. alata, L. pumila var. lanceolata and Labisia paucifolia using an ISSR assay. Fifty-eight primers were tested, among which 18 gave reliable polymorphic banding patterns; these yielded 264 polymorphic markers. A similarity ma...

Sequence-related amplified polymorphism (SRAP), amplified fragment length polymorphism (AFLP), and inter-simple sequence repeat (ISSR) markers were used to estimate the genetic diversity and relationships among Eucommia ulmoides cultivars in China. A total of 240, 192, and 150 DNA fragments were detected by 10 SRAP primer combinations, 10 AFLP primer combinations, and 10 ISSR primers, among whi...

29 Introduction 30 Materials and Methods 31 Results 32 Discussion 34 References 36 CHAPTER 3 Identification and pathogenicity of Graphium and Pesotum species from machete wounds on Schizolobium parahybum in Ecuador Abstract 43 Introduction 44 Materials and Methods 46 Results 49 Discussion 50 References 5343 Introduction 44 Materials and Methods 46 Results 49 Discussion 50 References 53 CHAPTER ...

Blumeria graminis f. sp. tritici, which causes wheat powdery mildew, is an obligate biotrophic pathogen that can easily genetically adapt to its host plant. Understanding the virulence structure of and genetic variations in this pathogen is essential for disease control and for breeding resistance to wheat powdery mildew. This study investigated 17 pathogenic populations in Sichuan, China and c...

This article refers to viewing the role of molecular markers during analyzing genome plants and their importance in plant biotechnology. In recent years, we observed techniques programs for improving breeding preserving genetic resources has been observed, biochemical indicators which represent basic material through determining diversity between genotypes it is never affected by external surro...

Twelve microsatellite markers were developed for population analyses of the fungal pathogen, Dothistroma septosporum. Intersimple sequence repeat polymerase chain reaction (ISSR-PCR) and an enrichment protocol (fast isolation by amplified fragment length polymorphism of sequences containing repeats [FIASCO]) were both used to identify 28 unique microsatellite regions in the genome. From 22 prim...