We developed a nicking endonuclease dependent DNA amplification (NDA), using Nt.BstNBI to catalyze single-stranded nick on double-stranded DNA, and Bst DNA polymerase to make extension while sealing the nick and displacing the downstream strand. The displaced single-stranded DNA thereby serves as template for primers hybridization and extension, resulting in exponential synthesis of target DNA ...

In this paper a passive bilateral tele-operation between two pneumatic actuators is presented. The co-ordinated system is controlled to behave as a rigid mechanical tool that interacts with the human and other environment inputs. The input human forces when applied via a force sensor, can be amplified through the tele-operator to provide assistance for the human operator to perform the task eit...

We present a method of rapid isothermal amplification of DNA without initial heat denaturation of the template, and methods and probes for (a) real-time fluorescence detection and (b) lateral flow detection of amplicons. Isothermal strand displacement amplification (iSDA) can achieve >10(9)-fold amplification of the target sequence in <20 minutes at 49 °C, which makes it one of the fastest exis...

The Extragalactic Lens VLBI Imaging Survey (ELVIS) searches for central images of lensed radio quasars, in order to measure the central density profiles of distant galaxies. Here we present sensitive multi-epoch Very Long Baseline Array (VLBA) observations of PMN J1838–3427 at 8 GHz, with a 1 σ noise level of 38 μJy beam. Based on the absence of a central image of the background source at this ...

Controlled release tablets containing 50 mg diclofenac sodium (DS) and 40% mastic with other natural additives were prepared. Drug release was examined and stability was studied using non-isothermal and isothermal thermogravimetric analysis (TGA). The bioavailability of two controlled release tablet formulations was studied and compared to that of commercial tablets, and rabbit stomachs were al...

Hierarchical clustering model has been extensively used in studies of the cosmological structure formation. In the hierarchical model, such as the standard Cold Dark Matter (CDM) dominated universe, the structures are formed in a bottom-up fashion. In this letter, we show that dark halos formed through the hierarchical clustering have non-isothermal structure and central density cusp shallower ...

We use the lubrication approximation to investigate the unsteady gravity-driven draining of a thin rivulet of Newtonian fluid with temperature-dependent viscosity down a substrate that is either uniformly hotter or uniformly colder than the surrounding atmosphere. First we derive the general nonlinear evolution equation for a thin film of fluid with an arbitrary dependence of viscosity on tempe...