Factors controlling the rate of oxidation of succinate were studied in mitochondria isolated from twenty-seven specimens of normal human liver obtained at operation. These were compared with mitochondria prepared from the livers of anesthetized and unanesthetized rats. Previously reported lower rates of succinate oxidation by human liver mitochondria were confirmed. This difference was no longe...

Bile secretion is regulated by different signaling transduction pathways including protein kinase C (PKC). However, the role of different PKC isoforms for bile formation is still controversial. This study investigates the effects of PKC isoform selective activators and inhibitors on PKC translocation, bile secretion, bile acid uptake, and subcellular transporter localization in rat liver, isola...

Cathepsin S was purified from bovine spleen by acid autolysis, (NH4)2SO4 fractionation and chromatography on CM-Sephadex C-50, CM-cellulose and activated-thiol-Sepharose. Cathepsin L was isolated from lysosomal fractions of rat liver, rat kidney and bovine liver. Generally, cathepsin L was bound tightly to CM-Sephadex C-50. Preparations of cathepsin L from rat liver, rat kidney and bovine liver...

background: chemical toxins are among the leading causes of liver disorders, inflammation and necrosis in hepatocytes. the aim of this study was to evaluate of protective effect of hydroalcoholic extract from rhizome of zingiber officinale l. (hez) on carbon tetrachloride-induced hepatic injury in male rat. material and methods: in this experimental studymale wistar rats (n=35, body weight 230-...

background: alcoholic mothers who consume ethanol give birth to infants with high percentage of hepatic diseases. alcohol can cause cellular damage in different tissues, including liver. finding a drug which is effective and efficient in reducing ethanol misuse consequences during pregnancy can assist the decrease of harmful effects of this habit. objective: this research aims to study the effe...

in pregnancy period, there is high risk of hepatic diseases and alcohol consumption increases such risk. some pregnant mothers are not able to quit the habit of drinking alcohol or they are unaware of its dangers. finding a drug which is effective and efficient in reducing ethanol misuse consequences during pregnancy can assist the decrease of harmful effects of this habit. the purpose of the c...

introduction cold plasma is a self-sterilized, painless, and non-contact method in surgeries. these properties allow it to be applied to the living tissues and heat-sensitive parts. the aim of this study was to design a new cold plasma producer device and evaluate the effects of cold argon plasma on decreasing the coagulation time of blood drop in vitro and that of the injured liver blood in vi...

The effects of different concentrations of the hydroalcoholic extract of dried powdered leaves of Cichorium intybus L., on CCl4-induced hepatotoxicity in vivo in rats and CCl4-induced cytotoxicity in isolated rat hepatocytes were investigated. Rats received different concentrations of the extract by i.p. injection for 3 consecutive days before the injection of (3ml/kg) CCl4 (i.p.). Twenty four ...

2-Amino-3-carboxymuconate-6-semialdehyde decarboxylase (ACMSD; EC 4.1.1.45) is one of the important enzymes regulating tryptophan-niacin metabolism. In the present study, we purified the enzyme from rat liver and kidney, and cloned the cDNA encoding rat ACMSD. The molecular masses of rat ACMSDs purified from the liver and kidney were both estimated to be 39 kDa by SDS/PAGE. Analysis of N-termin...

objective: crocin, the carotenoid isolated from saffron, has numerous medicinal properties which include anticancer and antioxidant activities. some antioxidants, such as carotenoids, can act as pro-oxidants at higher dosages and therefore induce tissue damage. in this situation antioxidant defense systems in the liver activate to prevent tissue damage. this study investigates the possible toxi...