BACKGROUND Hyperoxic preconditioning is known to protect the heart against necrosis and contractile dysfunction, but protection against arrhythmias has not been well characterized. OBJECTIVE The authors hypothesized that pre-exposure to normobaric hyperoxia (H) reduces ischemia and reperfusion-induced arrhythmias in isolated rat hearts. METHODS Following 60 and 180 min of hyperoxia treatmen...

ROSKOSKI, ROBERT, JR., RUTH I. MCDONALD, LAURA M. ROSKOSKI, WILLIAM J. MARVIN, AND KENT HERMSMEYER. Choline acetyltransferase activity in heart: evidence for neuronal and not myocardial origin. Am. J. Physiol. 233(6): H642-H646, 1977 or Am. J. Physiol.: Heart Circ. Physiol. 2(6): H642-H646, 1977. -Choline acetyltransferase (EC 2.3.1.6) catalyzes the following reaction: acetyl coenzyme A + choli...

objective: oxidative stress damages cells and brings about the pathogenesis of ischemia/reperfusion injury. this study was carried out to investigate the preconditioning and cardio protective potential effects of crocin and vitamin e by the enos and inos express gene in ischemia/reperfusion in rats. material & methods: male rats were divided into seven groups, namely: sham, control group and ex...

Abstract Introduction: Isolated perfused heart models such as perfusion and superfusion are commonly used for mammalian heart research. However, there are several fundamental limitations in the current techniques. In perfusion model, a suitable cannula is connected to the aorta and the perfusion is retrogradely performed. But, electrode displacement is a potential unwanted event resulted fr...

it has been known that cardiomyocytes possess a remarkable ability to downregulate their energy expenditure on restricted 02 supply. however it has also been speculated that an ability to suppress aerobic respiration and atp utilization could be a protective response to prolonged hypoxia. having the role of the cytochrome oxidase enzyme in mind, one can ask if its inhibition by using cyanide ha...

It has been hypothesized that free radical metabolism and oxygenation in living organs and tissues such as the heart may vary over the spatially defined tissue structure. In an effort to study these spatially defined differences, we have developed electron paramagnetic resonance imaging instrumentation enabling the performance of three-dimensional spectral-spatial images of free radicals infuse...

Substrate utilization by the isolated aerobic (Po2 500 mm Hg) and hypoxic (Po2 50 mm Hg) turtle (Pseudemys scripta) heart was evaluated by retrograde perfusion. During aerobic perfusion, the heart demonstrated active extraction and oxidation of free fatty acid (FFA) even in the presence of exogenous glucose. FFA metabolism was similar to that seen in the mammalian heart and was adequate to meet...

A new cyclodepsipeptide, designated roseocardin, was isolated from the culture broth of Trichothecium roseum TT103. Roseotoxin B and destruxins A and B were also isolated during the same procedure. The structure of roseocardin was determined by EI-MS, NMR and X-ray crystallographic analysis. Roseocardin as well as the other cyclodepsipeptides were shown to produce positive inotropic effects on ...

Isoprenaline (ISO) at high doses can cause severe stress to myocardium resulting in an infarct-like necrosis rats. However, its effects repeatedly low dose exposure on heart, kidney and aorta are still unclear. Hence, this study was aimed investigate the of repeated administration ISO organs rats by using Langendorff-perfused isolated hearts, ELISA kits, qPCR histopathology techniques. Male Wis...

The sequence of atrial natriuretic factor (ANF) has been determined, as well as the complete structure of the rat and human complementary DNA and gene. ANF and ANF messenger RNA are present not only in atria but also in ventricles. The circulating form of ANF has been identified as the C-terminal of the molecule, ANF (Ser 99-Tyr 126). The isolated secretory granules of rat atrial cardiocytes co...