A single site mutant of Bacillus subtilis with a streptovaricin-resistant RNA polymerase has been isolated; this mutation caused temperature-sensitive sporulation, but had no effect on vegetative growth. The mutant (ts710) temperature-sensitive period irreversibly affected the middle and late stages of sporulation. Mutant cells grown at the nonpermissive temperature exhibited abnormal serine pr...

We describe the recurrence of a cross-reactive idiotope (CRIAD8) in antibody responses to different epitopes, and explore factors leading to its dominance in some responses, but not others. Serological and genomic DNA analyses showed that CRIAD8 is a marker of the CRIa heavy chain variable region (VH) that encodes the majority of anti-p-azobenzenearsonate (anti-ABA) antibodies. The independence...

Twenty-nine mild asthmatics and 12 nonasthmatics were exposed to three films of varying emotional content to assess differential patterns of physiological response in the cardiac, ventilatory, and bronchial systems. Results indicated that only cardiac interbeat interval (IBI) and total respiratory resistance (RT) were reliable indices of reactivity. In a film of thematic relevance to asthmatics...

Background: We evaluated the carbapenem inactivation method (CIM) compared with the modified Hodge test (MHT) for the detection of carbapenemase-producing Gram-negative bacilli. Methods: A total of 61 isolates of carbapenemaseproducing Enterobacteriaceae (CPE: 14 KPC, 7 GES5, 8 NDM-1, 9 VIM-2, 9 IMP-1, and 14 OXA-48-like), 34 isolates of metallo-β-lactamase (MBL)-producing Pseudomonas spp. (14 ...

there has been a renewed interest in edible films made of renewable and natural polymers such as protein, polysaccharide and lipids. natural polymers derived from natural sources like food protein, offer the greatest opportunities because of their biodegradability and their ability to supplement nutritional value of foods. faba bean is a valuable source of protein and is cultivated in large qua...

The virulence index of three Meloidogyne incognita field isolates to the resistance gene Rk in cowpea was 0%, 75%, and 120%, with the index measured as reproduction on resistant plants as a percentage of the reproduction on susceptible plants. Continuous culture of the 75% virulent isolate on susceptible tomato for more than 5 years (about 25 generations) resulted in virulence decline to about ...

Disadvantageous hybridization favors the evolution of prezygotic isolating behaviors, generating a geographic pattern of interspecific mate discrimination where members of different species drawn from sympatric populations exhibit stronger preference for members of their own species than do individuals drawn from allopatric populations. Geographic shifts in species' boundaries can relax local s...

in pakistan besides pesticide contamination from agricultural field, the manufacturing industriesare also contributing relatively high quantities of toxic pesticides into the environment. these pesticides maybe toxic, mutagenic or carginogenic and may be bioaccumulated or biomagnified by the biota. because ofpersistent nature of pesticides, its removal from environmental system become difficult...