background: auto antibodies to zona-pellucida (aza) seem to be important autoantibodies implicated in reproduction, with substantial role in both endocrine and reproductive functions of the human ovary. there are some debates on the relation of aza with infertility, repeated in vitro fertilization (ivf) attempts, and outcome of it. objective: in this study, we assessed the presence of aza in th...

background: there is increasing evidence that psychological factors like anxiety and depression can affect ivf/icsi treatment results. objective: this study aimed to clarify the role of women’s anxiety and depression on the outcome of art cycles using intra cytoplasmic sperm injection (icsi). materials and methods: this was a prospective pilot study. one hundred six (106) consecutive women unde...

Abstract Study question Will use of surgically retrieved Testicular Sperms (TESA) in Intra-Cytoplasmic Sperm Injection (ICSI) cycles increase embryo aneuploidy? Summary answer Use TESA sperms for ICSI seems safe and doesn’t aneuploidy. performed various indications also showed no variation with What is known already Invention improved surgical sperm retrieval techniques have helped men severe m...

background: testis tissue xenografting and the resultant sperm in a xenograft may provide a unique approach to rescue the genetic material of males that die prematurely and is a model for the study of human spermatogenesis and can represent an alternative approach for fertility preservation in cancer patients. this study was aimed to evaluate the xenogenic dog sperm in formation of male pronucl...

background: pregnancy is a successful transplantation. the factors evading rejection of the fetus are poorly understood. recently an interest has grown, in hla-g molecules as one of these factors. since these antigens are mainly expressed on the surface of cytotro-phoblasts that are in direct contact with maternal tissues, it has been suggested that these molecules may have a role in induction ...

Sodium-hydrogen exchanger as a channel for regulation of intracellular pH might be a crucial modulator of sperm capacitation and motility. Three members of this family have been identified in spermatozoa. A novel protein testis-specific sodium-hydrogen exchanger named mtsNHE was cloned in the present study. The mtsNHE localizing on principle piece of sperm flagellum contained 12 predicted trans...

Microinjection of cytosolic sperm extracts into unfertilized golden hamster eggs caused a series of increases in cytoplasmic free calcium, Ca2+i, and membrane hyperpolarizing responses, HRs. These HRs and Ca2+i transients are similar to those seen during in vitro fertilization of hamster eggs. The sperm factor that is responsible for causing these effects appears to be of high molecular weight ...

Reproductive failure is one of the most important issues for the population at age of procreation and approximately 15% of the couples who try to conceive a baby encounter reproductive difficulties. In this study, we used multicolor fluorescent in situ hybridization (FISH) probes for chromosomes 13, 18, 21, X and Y to evaluate the aneuploidy incidence in sperm cells. The study group included 35...

Abstract Study question In men with Raised Sperm DNA Fragmentation Index (DFI), Would advance sperm preparation techniques at Intra-Cytoplasmic Injection (ICSI) alter embryo euploidy status? Summary answer raised DFI, use of selection are safe and do not appear to ploidy status the embryos What is known already MACS (Magnetic Activated Cell Sorting), Microfluidic Sorting (MF), surgically retrie...