We assayed serum and urine specimens for amylase activity by the nephelometric (I),dyed-starch (Amylochrome) (II), and mayloclastic (III) techniques. For serum, the correlation coefficients of the regression lines were: I vs. II, 0.978 (n = 106); I vs. III, 0.736 (n = 110); and II vs. III, 0.739 (n = 108). For urine, they were I vs. II, 0.938 (n = 49); and I vs. III, 0.752 (n = 46). Because cal...

the experiment was conducted to determine α-amylase activity and the effect of seven plant species extracts including punica granatum l. (punicaceae), rheum officinale b. (polygonaceae), rhus coriaria l. (anacardiaceae), artemisia sieberi b. ( compositae), peganum harmala l. (nitrariaceae) , datura stramonium l. (solanaceae) and thymus vulgaris l. (lamiaceae) on α-amylase activity of four store...

the experiment was conducted to determine α-amylase activity and the effect of seven plant species extracts including punica granatum l. (punicaceae), rheum officinale b. (polygonaceae), rhus coriaria l. (anacardiaceae), artemisia sieberi b. ( compositae), peganum harmala l. (nitrariaceae) , datura stramonium l. (solanaceae) and thymus vulgaris l. (lamiaceae) on α-amylase activity of four store...

Pancreatic exocrine secretion is affected by galanin, but the mechanisms involved are unclear. We aimed to determine the effect and elucidate the mechanism of action of exogenous galanin on basal and stimulated pancreatic amylase secretion in vitro. The effect of galanin on basal-, carbachol-, and caerulein-stimulated amylase secretion from isolated murine pancreatic lobules was measured. Carba...

Diabetes mellitus is one of the most common endocrine diseases and its type 2 is the major form of diabetes, accounting for 90% of cases worldwide. One of the complications of diabetes is post-prandial hyperglycemia (PPHG). The inhibition of carbohydrate hydrolyzing enzymes such as α-amylase can be an important in the control of blood glucose levels and managing PPHG in patients with type 2 dia...

The presence of ar-amylase in liver tissue has been reported previously by several investigators (l-3). In the liver from rats deprived of food, the enzyme is found primarily in the microsomal fraction (4) whereas in fed animals a considerable proportion is bound to glycogen (5). In perfused liver systems, amylase activity is secreted into the perfusion medium under conditions in which other en...

Kernels of Klages barley (Hordeum vulgare L.) were germinated for 1 to 4 days on moist sand at 18 degrees C. Representative kernels from each time period were dissected to give the following fractions: scutellum, subscutellar endosperm, aleurone-scutellum interface, remaining aleurone, subaleurone endosperm, and core endosperm. These tissues were analyzed for alpha-amylase components by isoelec...

We developed a kinetic procedure for determination of amylase activity in serum by use of nephelometric measurements. Light scattering from the substrate, a stable suspension of starch, is decreased as amylase hydrolyzes the starch to soluble fragments. Values obtained for serum amylase correlate closely with those determined by a method in which a starch-dye complex is used. Precision of the n...

Considering the weak gel of tapioca starch and generally incomplete gel-advancing mechanism α-amylase modification, two commercial α-amylases (named A- N-amylase) with different reaction patterns were used to improve strength cross-linked starch. SEM showed that A-amylase exhibited surface erosion pattern, compared an extensive pattern N-amylase. XRD, SAXS, HPGPC-MALLS-RI revealed mainly attack...

An elderly lady with amylase-rich ascites is presented, whose isoenzyme estimation revealed salivary type amylase. Tumour hyperamylasemia is an important group among the nonpancreatic causes of elevated amylase.