We present a theoretical and experimental study of analyte preconcentration via peak mode isotachophoresis (ITP). We perform perturbation analysis of the governing equations that includes electromigration, diffusion, buffer reactions, and nonlinear ionic strength effects. This analysis relaxes the inherent numerical stiffness and achieves a fast solution to the transient sample evolution proble...

We present and demonstrate a novel technique for the purification of nucleic acids from biological samples using isotachophoresis (ITP). We demonstrate a simple and rapid method to achieve ITP-based extraction, preconcentration, and purification of DNA from nanoliter volumes of whole blood. We show that ITP purification yields genomic DNA samples which can be quantitated with fluorescence measu...

BACKGROUND AND PURPOSE Purinergic nucleoside inosine elicits protection and regeneration during various injuries. The purpose of this study was to examine the protective effects of inosine against cerebral ischemia. METHODS Adult Sprague-Dawley rats were anesthetized. Inosine, hypoxathine, or vehicle was administered intracerebroventricularly before transient right middle cerebral artery occl...

BACKGROUND There is some evidence that when mental health commitment hearings are held in accordance with therapeutic jurisprudence principles they are perceived as less coercive, and more just in their procedures leading to improved treatment adherence and fewer hospital readmissions. This suggests an effect of the hearing on therapeutic relationships. We compared working alliance and interper...

Background and Objectives. A recent evaluation carried out by the Associazione Italiana di Ematologia e Oncologia Pediatrica (AIEOP) about practice management of acute childhood idiopathic thrombocytopenic purpura (ITP) revealed a remarkable difference of behaviors among the different AIEOP centers. A need for common practice guidelines for this frequent illness arose from this observation. Our...

Reports of the incidence of ITP are few and their methodology is variable. Accurate estimates of the incidence of immune thrombocytopenic purpura (ITP) are important to understand the medical and public health impact of the disease. To critically review all published reports on the incidence of ITP in children and adults, all articles identified on the Medline database (searched January 1, 1966...

A minority or children with idiopathic or immune thrombocytopenic purpura (ITP) have the rerractory chronic rorm with bleeding problems (stage III or IV). The aim or this article is to provide an update on the immunopathogenesis and management or children with bleeding and severe refractory lIP. The man~lgement of such patients. according to a st~lging guideline. is described. I{ecent ciinic~11...

BACKGROUND Many people now take multiple medications on a long-term basis to manage health conditions. Optimising the benefit of such polypharmacy requires tailoring of medicines use to the needs and circumstances of individuals. However, professionals report barriers to achieving this in practice. In this study, we examined health professionals' perceptions of enablers and barriers to deliveri...

University, Jinan, China; Department of Chemical and Biological Engineering, Chalmers University of Technology, Gothenburg, Sweden; Department of Clinical Laboratory, and Department of Pediatrics, Qilu Hospital, Shandong University, Jinan, China; Key Laboratory of Cardiovascular Remodeling and Function Research, Chinese Ministry of Education and Chinese Ministry of Health, Jinan, China; and Dep...