A particulate subcellular fraction from Escherichia coli K-12 induced in anaerobic sn-glycerol 3-phosphate (G3P) dehydrogenase and fumarate reductase can catalyze under anaerobic conditions the transfer of hydrogens from G3P to fumarate, with attendant generation of high-energy phosphate. The phsophorylation process is more sensitive than the transhydrogenation process to inhibition by the dete...

Phosphorus (P) regeneration and transformation in the oceanic water column and in marine sediments depends on the chemical nature of the sinking particulate P pool. For the first time, we have characterized the molecular composition of this pool, in various oceanic settings and water depths, using P nuclear magnetic resonance (NMR) spectroscopy. Both inorganic P (orthophosphate, pyrophosphate, ...

High-frequency sampling of two major stream inflows to a large eutrophic lake (Lake Rotorua, New Zealand) was conducted to measure inputs of total suspended sediment (TSS), and fractions of nitrogen and phosphorus (P). A total of 17 rain events were sampled, including three during which both streams were simultaneously monitored to quantify how concentration-discharge (Q) relationships varied b...

Phosphorus (P) and potassium (K) are two major nutrients for agricultural productivity sustainability. The spatial variability maps of soil phosphorus content in surfacesoils collected through grid sampling technique were developed using geo-spatial technology Indian Council Agricultural Research-Indian Research Institute (ICAR-IARI) farm, New Delhi, India. Soil available P (NaHCO3-P) P-fractio...

Isolated mouse liver mitochondria were incubated in two types of P(32)-labelled sucrose-phosphate buffers. The first contained no added ATP or oxidizable substrate. The second contained added ATP. Samples were taken at specified times, up to 60 minutes, and analyses were made of the mitochondrial TCA-soluble inorganic P(32) and the total mitochondrial residue P(31) and P(32). The results of the...

Because of the similarity in structure between diiodotyrosine and thyroxine, Harington and Barger (1) suggested that the former is the precursor of thyroxine in the thyroid gland. The formation of thyroxine from diiodotyrosine has been demonstrated lately in vitro (2, 3). Recent data (4, 5) obtained with radioiodine used without carrier indicate the rates of incorporation of iodine into the var...

In Rhodospirillum rubrum, inorganic pyrophosphatase activity is observed in both the cytoplasmic and membrane fractions. The soluble enzyme accounts for about 80% of the total activity in crude extracts, and is the subject of this report. Zn(2+) is required for both activity and stability of the enzyme, which has a molecular weight of approximately 90,000 (gel-filtration determinations). The su...

A long-term fertilization experiment was conducted to examine the effects of different fertilization practices on nematode community composition within aggregates in a wheat-maize rotation system. The study was a randomized complete block design with three replicates. The experiment involved the following four treatments: no fertilizer, inorganic N, P and K fertilizer (NPK), NPK plus manure (NP...

The utilization of expressed plant juice for determining the chemical constituents of a plant is becoming increasingly important. It has the advantage of presenting the products of metabolism in a state more similar to that found in living tissue than any of the more commonly used extraction methods. Conditions of state and equilibrium, however, can probably never be identical for juice within ...

For fast detection of inorganic phosphorus fractions and their phosphorus contents in soil, a method employing near-infrared spectroscopy (NIRS) combined with partial least squares (PLS) and least squares support vector machine (LS-SVM) was proposed. Fifty soil samples for each of iron phosphate, magnesium phosphate, calcium phosphate and aluminum phosphate, with application rates of phosphate ...