Chlamydomonas reinhardtii mutants defective in the chloroplast ATP synthase are highly sensitive to light. The ac46 mutant is affected in the MDH1 gene, required for production or stability of the monocistronic atpH mRNA encoding CF(O)-III. In this and other ATP synthase mutants, we show that short-term exposure to moderate light intensities-a few minutes-induces an inhibition of electron trans...

glassy carbon (gc) is the most commonly used carbon-based electrode in the analytical laboratory. because of the high background current and low electrode response, modification of this electrode can be done by various materials and techniques. an ionic liquid (il), 1-methyl-3-(3-trimethoxysilyl propyl) imidazoliumbis (trifluoromethylsulfonyl) imide, was covalently cross linked onto the gc surf...

Tanshinone IIA exerts anti-inflammatory effects and influences electron transfer reaction in mitochondria. In the present study, we demonstrated that tanshinone IIA increased intracellular production of reactive oxygen species (ROS), which in turn induces heme oxygenase-1 (HO-1) expression in RAW 264.7 macrophages. Tanshinone IIA inhibited COX-2 and iNOS expression in lipopolysaccharide-activat...

Marine luminous bacteria were used to monitor toxicity of alpha(Am-241, U-235+238) and beta(tritium) radionuclide solutions. Increase or inhibition of bacterial luminescence was observed under exposure to radionuclides. Radiation toxicity of Am and chemical toxicity of U were demonstrated. Effects of U were similar to those of stable heavy metals: sensitivy was about 10M. Sensitivity of the bac...

PINK1 is mutated in Parkinson's disease (PD), and mutations cause mitochondrial defects that include inefficient electron transport between complex I and ubiquinone. Neurodegeneration is also connected to changes in lipid homeostasis, but how these are related to PINK1-induced mitochondrial dysfunction is unknown. Based on an unbiased genetic screen, we found that partial genetic and pharmacolo...

Mitochondrial respiration is the predominant source of ATP. Excessive rates of electron transport cause a higher production of harmful reactive oxygen species (ROS). There are two regulatory mechanisms known. The first, according to Mitchel, is dependent on the mitochondrial membrane potential that drives ATP synthase for ATP production, and the second, the Kadenbach mechanism, is focussed on t...

Gap junctions provide direct electrical and biochemical communication between cardiomyocytes in the heart. Connexin40 (Cx40) is the major connexin in the atria of the heart and little is known regarding its regulation. Thus, the goal was to investigate the regulation of Cx40 in both physiological and pathophysiological conditions. The first objective of this thesis was to determine whether Cx40...

The ion/ion reactions of several dozen reagent anions with triply protonated cations of the model peptide KGAILKGAILR have been examined to evaluate predictions of a Landau-Zener-based model for the likelihood for electron transfer. Evidence for electron transfer was provided by the appearance of fragment ions unique to electron transfer or electron capture dissociation. Proton transfer and ele...

europium (eu) doped zno microsphere based nano-sheets were synthesized through hydrothermal method. effects of different concentrations of europium on structural and optical properties of zno nano-sheets were investigated in detail. prepared un-doped and eu-doped zno samples were characterized using x-ray diffraction (xrd), energy dispersive x-ray spectroscopy (edx), scanning electron microscop...