نتایج جستجو برای: indirect plate elisa

تعداد نتایج: 195615  

2015
Luigi Bertolotti Elvira Muratore Chiara Nogarol Claudio Caruso Laura Lucchese Margherita Profiti Laura Anfossi Loretta Masoero Stefano Nardelli Sergio Rosati

BACKGROUND Bovine herpesvirus 1 (BoHV1) is a member of the viral subfamily of Alphaherpesvirinae that infects various species, including cattle, sheep, and goats. The virus causes infectious bovine rhinotracheitis (IBR), which is included in a European list of diseases that may require control and eradication programs. The lack of confirmatory tests affects the validity of diagnostic tools, esp...

Journal: :Journal of clinical microbiology 1984
M M Chengappa G R Carter W E Bailie

A rapid, simple, and accurate counterimmunoelectrophoresis (CIE) technique was developed and compared with the indirect hemagglutination and rapid plate agglutination techniques for serotyping cultures of Pasteurella haemolytica. The CIE test had 100% correlation with the conventional indirect hemagglutination test and, after serum absorption, correctly identified cultures representing the 12 e...

2017
Gábor Gellért

In this study a robust and simple classification into different stages of pemphigus, based on a set of therapeutic benchmarks including a definition of disease activity, is proposed. This staging method was validated against ELISA titres for autoantibodies against desmoglein-1 and -3. The proposed staging method was applied a priori to five newly diagnosed pemphigus patients in the course of th...

Journal: :Journal of clinical microbiology 1997
T Aoyama T Kato Y Takeuchi K Kato K Morokuma T Hirai

We developed a particle agglutination test (KPA) with poly(gamma-methyl L-glutamate) as the solid particle for measurement of pertussis toxin (PT) antibody. In this study, KPA was assessed as a means of serodiagnosing pertussis, and the results were compared with those of indirect enzyme-linked immunosorbent assay (indirect ELISA) and the microagglutination test. First, four serum samples were ...

H. Dadras H. Ghadakchi S.A. Pourbakhsh, S.M.H. Hosseini

An indirect enzyme–linked immunosorbent assay (ELISA) was developed for screening of antibody to avian infectious bronchitis virus (IBV). Antigen was prepared from whole-purified IBV Massachusetts serotype (BR 801 strain). Optimum dilution with minimum background for antigen concentration, rabbit anti-chicken conjugate and sera in developed ELISA was determined 0.1μg/ml, 1:3000 and 1:100, respe...

Journal: :Journal of clinical microbiology 1984
S H Cheeseman G V Doern R A Ferriani M W Keville B R McGraw J A Stewart

By the use of two reference procedures, an indirect hemagglutination assay and a complement fixation test, the presence or absence of cytomegalovirus (CMV) antibody was determined for 221 human sera. Ninety-nine sera (44.8%) were found to contain CMV antibody. The remaining 122 sera (55.2%) lacked detectable CMV antibody. These same sera were then analyzed by two recently introduced, commercial...

2016
Yaseen M. Arabi Ali H. Hajeer Thomas Luke Kanakatte Raviprakash Hanan Balkhy Sameera Johani Abdulaziz Al-Dawood Saad Al-Qahtani Awad Al-Omari Fahad Al-Hameed Frederick G. Hayden Robert Fowler Abderrezak Bouchama Nahoko Shindo Khalid Al-Khairy Gail Carson Yusri Taha Musharaf Sadat Mashail Alahmadi

We explored the feasibility of collecting convalescent plasma for passive immunotherapy of Middle East respiratory syndrome coronavirus (MERS-CoV) infection by using ELISA to screen serum samples from 443 potential plasma donors: 196 patients with suspected or laboratory-confirmed MERS-CoV infection, 230 healthcare workers, and 17 household contacts exposed to MERS-CoV. ELISA-reactive samples w...

2017
Kusnoto Setiawan Koesdarto Sri Mumpuni R. Heru Prasetyo

Aim The aim of this study was to detect specific immunoglobulin (Ig) that could be used to determine monoclonal antibody in conjugate-making an effort for the indirect enzyme-linked immunosorbent assay (ELISA) diagnostic kit of toxocariasis in human. Materials and Methods The study was conducted to assess the Ig profile, based on ELISA-isotyping, in mice infected with second stage larvae eggs...

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