background: cystic hydatid disease is an important zoonosis, affecting humans and animals and is a significant public health and economic problem throughout the world and iran. since extraction of dna from the parasite is a primary and crucial step which has a principal effect on pcr results, in the current study five simple methods for dna extraction from protoscoleces of echinococcus granulos...

Two new species isolated from plant leaves belonging to Talaromyces section Talaromyces are reported, namely T. neofusisporus (ex-type AS3.15415 (T) = CBS 139516 (T)) and T. qii (ex-type AS3.15414 (T) = CBS 139515 (T)). Morphologically, T. neofusisporus is featured by forming synnemata on CYA and YES, bearing appressed biverticillate penicilli and smooth-walled fusiform conidia about 3.5-4.5 × ...

Babesia gibsoni is a tick-borne apicomplexan parasite of dogs that often causes fever and hemolytic anemia with highly variable clinical outcome. In this study, we sequenced the 254bp Internal Transcribed Spacer 1 region (ITS1) of 54 B. gibsoni isolates from 14 different geographical regions of Japan. The 54 isolates shared high sequence identity with each other and with B. gibsoni isolates rep...

Macrogyrodactylus spp. from the gills of Clarias gariepinus in Zimbabwe and Kenya, and C. anguillaris in Senegal were identified using haptoral sclerite morphology and by sequencing the nuclear ribosomal DNA internal transcribed spacers (ITS) 1 and 2, partial 18S and the complete 5.8S rRNA gene. A molecular phylogeny was constructed using all sequenced Macrogyrodactylus species to date. Based o...

The aim of the study was the molecular analysis of ITS1/5.8S rRNA/ITS2 region of Trichomonas gallinae isolates from racing pigeon lofts in Upper Silesia, Poland. The analysed region is very useful for the taxonomy of the Trichomonadidae family and indicates the possible existence of different genotypes or species within the T. gallinae. A comparison of the complete ITS1-5.8S-ITS2 region of obta...

The specific identification of Lymnaeid snails is based on a comparison of morphological characters of the shell, radula, renal and reproductive organs. However, the identification is complicated by dissection process, intra and interspecific similarity and variability of morphological characters. In the present study, polymerase chain reaction and restriction fragment length polymorphism (PCR-...

Parabronema skrjabini is a spirurid nematode of the family Habronematidae that lives in the abomasum of ruminants such as sheep and goats. The purpose of this study was to investigate the molecular aspects of Parabronema skrjabini in sheep. The worms were collected from sheep in Sanandaj (west of Iran). The first internal transcribed spacer (ITS) of ribosomal DNA (rDNA) nucleotide fragments of ...

Mastitis is an economically important disease in which fungi belonging to the genus Candida may participate as etiological agents. This study focused on determining the frequency of fungal isolation and differentiation of fungal species isolated from milk of mastitic cows. Sixty-six milk samples from mastitic cows were studied, and 55 strains of fungi were isolated. Seven different species clas...

BACKGROUND We evaluated the genetic diversity of samples identified morphologically as Fasciola spp. from sheep, cattle and goat from Kermanshah Province, western Iran using PCR-RFLP method. METHODS We used PCR-RFLP analysis of ribosomal ITS1 fragment using RsaI restriction enzyme to investigate the genetic characteristics of Fasciola species obtained from different hosts (16 sheep, 28 cattle...