Biophysical stimuli may be an effective therapy to counteract age-related changes in bone structure that affect the primary stability of implants used in joint replacement or fracture fixation. The influence of controlled mechanical loading on osseointegration was investigated using an in vivo device implanted in the distal lateral femur of 12 male rabbits. Compressive loads (1 MPa, 1 Hz, 50 cy...

objective: amphotericin b (amb) is an ancient antifungal drug and proper for treatment of systemic fungal infection. unfortunately, this antibiotic has shown some side effects such as nephrotoxicity. therefore, recently new amb formulated has been prepared for reduced toxicity and increased effectiveness. materials and methods: amb nanocapsules prepared from polymers such as poly d, l-lactide-...

We propose a culture-free approach to osteochondral repair with minced autologous cartilage fragments loaded onto a scaffold composed of a hyaluronic acid (HA)-derived membrane, platelet-rich fibrin matrix (PRFM) and fibrin glue. The aim of the study was to demonstrate in vitro the outgrowth of chondrocytes from cartilage fragments onto this scaffold and, in vivo, the formation of functional re...

introduction: the spermatogonial stem cells (sscs) form the basis of spermatogenesis. in vitro culture of germ cells is useful for the purification and increasing of sscs for transplantation. in the present study, we examined: in vitro the effects of co-culture with sertoli cells, gdnf, scf and gm-csf on efficiency of spermatogonial cells colony formation and spermatogenesis induction in the re...

Despite promising preclinical outcomes in animal models, a number of challenges remain for human clinical use. In particular, expanding a large number of endothelial progenitor cells (EPCs) in vitro in the absence of animal-derived products is the most critical hurdle remaining to be overcome to ensure the safety and efficiency of human therapy. To develop in vitro culture conditions for EPCs d...

the aim of the present study was to develop exemestane loaded polymeric nanoparticles for improved oral bioavailability of exemestane. exemestane loaded nanoparticles were prepared by solvent displacement method with eudragit rl 100 and eudragit l 100 as polymers and pluronic® f-68 as surfactant. the influence of various formulation factors (drug: polymer ratio and concentration of surfactant) ...

To characterize the replicative capacity of human central memory (T(CM)) CD4 T cells, we have developed a defined culture system optimized for the ex vivo expansion of Ag-specific CD4(+) T cells. Artificial APCs (aAPCs) consisting of magnetic beads coated with Abs to HLA class II and a costimulatory Ab to CD28 were prepared; peptide-charged HLA class II tetramers were then loaded on the beads t...

PURPOSE To assess the potential of an MRI gene reporter based on the ferritin receptor Timd2 (T-cell immunoglobulin and mucin domain containing protein 2), using T1- and T2-weighted imaging. METHODS Pellets of cells that had been modified to express the Timd2 transgene, and incubated with either iron-loaded or manganese-loaded ferritin, were imaged using T1- and T2-weighted MRI. Mice were als...