urease was immobilized on platinum electrode both by chemical binding and electropolymerization.the conductometric urea biosensor thus prepared showed a detection limit of 4.9×10-5 m and linear dynamic range from 4.9×10-5 to 5.8×10-3 m for urea concentration when the enzyme is covalently immobilized on pt electrodes. conductometric transducers respond to the changes in ionic strength thereby le...

Enzyme cost is the major problem for industrial scale application. Immobilization is a promising approach to moderate the enzyme cost factor and increase its stability and activity. In this study, sol-gel method was used to prepare the immobilization platform and entrapped lipase as one of the most used enzyme in dairy processing, cosmetics and pharmaceutical industries. Lipase from Candida rug...

We expressed and purified a recombinant P. pyralis luciferase with N-terminal His-tags. The silanized Ni or Cu-loaded magnetic particles were prepared and used to assemble the His-tagged P. pyralis luciferase. This enzyme immobilized on functionalized magnetic nanoparticles (MNPs) via electrostatic interactions of His-tag with Ni2+/Cu2+ ions on the surface of MNPs using si...

This communication describes the use of a molecular hydrogel to immobilize enzymes for catalyzing reactions in an organic solvent to attain superactivity and exceptional stability.

We describe an electrochemical oxidative desorption of benzenediazonium-grafted organic layers and immobilized proteins on the layers from indium-tin-oxide electrode surfaces.

Bioelectroremediation (BER) uses electrical current to stimulate catabolism of environmental pollutants, such as petroleum spills. However, applying soil may have adverse effects on the microorganisms involved in degradation. We identify and evaluate BER capabilities a strain Aspergillus niger obtained from petroleum-contaminated soil. Spores this immobilized alginate spheres (2 g) were mixed w...

Bioremediation is a low-cost, clean, and environmentally friendly method in managing marine pollution. Despite its great potential, bioremediation has own challenges. As an open system, limited nutrients fluctuating environmental conditions the ocean affect metabolism of degrading microorganisms, thus influencing biodegradation rate. Multiple strategies have been employed to enhance rate at var...

The biogenous iron oxide (BIO) from Leptothrix ochracea was transformed to an organic-inorganic hybrid support to prepare an excellent immobilized enzyme showing high catalytic performance.