The application of the climatic chamber presented in this paper to assess storage stability immobilized lipases is a new approach characterized by potential unifying study conditions biocatalysts created various laboratories. data achieved from storing chambers may be crucial for chemical and pharmaceutical industry. Our describes developed protocols immobilization via interfacial activation li...

The strong catalytic performance, eco-friendly reaction systems, and selectivity of enzyme-based biocatalysts are extremely interesting. Immobilization has been shown to be a good way improve enzyme stability recyclability. Chitosan-incorporated metal oxides, among other support matrices, an intriguing class matrices for the immobilization various enzymes. Herein, cross-linked chitosan/zinc oxi...

Although several powerful methods exist for the redesign of enzyme structure and function these are typically limited to the 20 most abundant proteinogenic amino acids. The use of chemical modification overcomes this limitation to allow virtually unlimited alteration of amino acid sidechain structures. If heterogeneous mixtures of enzyme products are to be avoided, however, the required chemist...

The use of biocatalysts for the industrial synthesis of chemicals has been attracting much attention as an environment-friendly synthetic method. Microbial cells play a leading role in 'chemo-enzymatic synthesis' because of their great diversity. Several microbes with unique catalytic abilities have been found through intensive screening and put to practical use. Besides, advanced molecular bio...

Parallel interconnected kinetic asymmetric transformations were performed in order to obtain enantioenriched derivatives starting from a set of racemic or prochiral compounds. Thus, in a one-pot reaction using two redox biocatalysts (a BVMO and an ADH) and a catalytic amount of cofactor that acts as a mediator, enantioenriched ketones, sulfoxides, and sec-alcohols were concurrently obtained in ...

The general approaches to enzymatic synthesis of semisynthetic betalactam antibiotics were developed. The principal scheme includes the enzymatic transformation of natural antibiotics to key amino acids and following their acylation to produce the semisynthetic betalactams. Ten various biocatalysts are enough for production of all semisynthetic betalactams according to the scheme. The physico-c...

Various furans are considered as valuable platform chemicals as they can be derived from plant biomass. Yet, for their exploitation, follow-up chemistry is required. Here we demonstrate that Baeyer-Villiger monooxygenases (BVMOs) can be used as biocatalysts for the selective oxidation of several furans, including 5-(hydroxymethyl) furfural (HMF) and furfural. A total of 15 different BVMOs were ...

Biocatalysts, especially enzymes, have the ability to catalyze reactions with high product selectivity, utilize a broad range of substrates, and maintain activity at low temperature and pressure. Therefore, they represent a renewable, environmentally friendly alternative to conventional catalysts. Most current industrial-scale chemical production processes using biocatalysts employ soluble enzy...