The DNA-[N 6-adenine]-methyltransferase (Dam MTase) of phage T4 catalyzes methyl group transfer from S-adenosyl-l-methionine (AdoMet) to the N6-position of adenine in the palindromic sequence, GATC. We have used a gel shift assay to monitor complex formation between T4 Dam and various synthetic duplex oligonucleotides, either native or modified/defective. The results are summarized as follows. ...

Dam break analysis plays a very important role in the risk assessment of dams. Large dams pose significantly higher risks to the people and property downstream of the dam as compared to smaller dams. So the flooding risk due to the possible failure of large dams is more crucial. The simulation of exceptional events characterized by high hydraulic and hydro-geologic risk is an actual problem for...

Regulated expression of the Escherichia coli dam gene has been achieved with the araBAD promoter lacking a ribosome binding site. Cultures of dam mutants containing plasmid pMQ430 show no detectable methylation in the absence of arabinose and complete methylation in its presence. Dam methyltransferase is a substrate for the Lon protease.

Abstract The challenges behind this research were encountered while drilling into the Ilam, Mauddud, Gurpi, and Mishrif Formations, where severe instability-related issues observed across weaker formations above reservoir intervals. In paper, geomechanical parameters carried out to determine optimum mud weight windows safe deviation trajectories using parameters. We propose a workflow equivalen...

Invasion of intestinal epithelial cells is a critical step in Salmonella infection and requires the expression of genes located in Salmonella pathogenicity island 1 (SPI-1). A key factor for SPI-1 expression is DNA adenine (Dam) methylation, which activates synthesis of the SPI-1 transcriptional activator HilD. Dam-dependent regulation of hilD is postranscriptional (and therefore indirect), ind...

Salmonella strains that lack or overproduce DNA adenine methylase (Dam) elicit a protective immune response to different Salmonella species. To generate vaccines against other bacterial pathogens, the dam genes of Yersinia pseudotuberculosis and Vibrio cholerae were disrupted but found to be essential for viability. Overproduction of Dam significantly attenuated the virulence of these two patho...

Objective. To investigate the frequency and influencing factors of rubber dam usage for endodontic procedures among general dentistry practitioners and specialized practitioners (endodontist) in Tianjin. Methods. Three hundred questionnaires were distributed among practitioners from 3 different types of medical institutions in Tianjin. Data were collected and analysed using Chi-square tests. Re...

LIM-kinases (LIMKs) play crucial roles in various cell activities, including migration, division, and morphogenesis, by phosphorylating and inactivating cofilin. Using a bimolecular fluorescence complementation assay to detect the actin-cofilin interaction, we screened LIMK1 inhibitors and identified two effective inhibitors, damnacanthal (Dam) and MO-26 (a pyrazolopyrimidine derivative). These...

Leakage is a major safety issue that, if left unchecked, may result in dam failure by various mechanisms. There is enormous pressure on dam operators to repair leaks without significant delays. Frequently, the need to reduce the risk of failure or control water loss has led to costly remedial repairs that are planned and executed without a complete understanding of the problem. A lack of approp...

How to accurately determine the mechanical parameters of rockfill is one of the key issues of concrete-face rockfill dams. Parameter back-analysis using internal or external monitoring data has been proven to be an efficient way to solve this problem. However, traditional internal or external monitoring methods have limitations in efficiency and long-term monitoring. In this paper, the displace...