An indirect immunofluorescence test with total anti-human immunoglobulin conjugate (IgG,A,M-IIF) can be used for joint detection of immunoglobulin A (IgA) and IgM antibodies, provided serum IgG is previously absorbed with anti-human IgG. To determine the validity of the IgG,A,M-IIF assay with absorbed sera, the results obtained were compared with those obtained by methods routinely used for the...

We describe new methods for the detection of immunoglobulin G (IgG) and IgM rubella-specific antibodies in serum. The IgG assay was based on a solid-phase rubella antigen immobilization approach, and the IgM assay was based on the IgM capture assay principle. Both assays used biotinylated antibodies (anti-human IgG and antirubella monoclonal antibody, respectively). The tracer system was based ...

Hantaan virus is the causative agent of severe hemorrhagic fever with renal syndrome. Clinical surveillance for Hantaan virus infection is unreliable, and laboratory verification is essential. The detection of virus-specific immunoglobulin M (IgM) and IgG in serum is most commonly used for the diagnosis of hantavirus infection. Testing of oral fluid samples instead of serum offers many advantag...

We are in agreement with the reported usefulness, in Taiwan, of the rapid, point-of-care laboratory diagnosis of dengue virus (DENV) infection employing the commercial non-structural protein 1 (NS1) Ag STRIP (Bio-Rad Laboratories, Maines-la-Couqette, France). In endemic areas, concurrent testing for DENV NS1 antigen and IgG and IgM, if accompanied by an estimation of platelet counts, would ensu...

OBJECTIVES To evaluate the role of serum IgG, IgM and IgA anti-dsDNA antibody isotypes in the diagnosis of systemic lupus erythematosus (SLE), and their association with clinical features and disease activity, in a large cohort of SLE patients. METHODS Sera of 200 SLE patients (mean age 34±10.3 years; 26 male and 174 female; median duration of disease 115 months, range 7-378), and of 206 cont...

Commercial immunoassays for detecting IgG and IgM antibodies against Epstein-Barr virus (EBV), viral capsid antigens (VCA), and IgGs toward EBV nuclear antigen-1 (EBNA-1) are routinely used in combination to categorize EBV infection status. In this study, we evaluated the performances of the Architect EBV VCA IgG, VCA IgM, and EBNA-1 IgG chemiluminescent microparticle assays (CMIAs) in EBV sero...

Коронавирусная болезнь (COVID-19) — инфекционное заболевание, которое вызывает недавно обнаруженный коронавирус, названный SARS-CoV-2. болезнь, вызванная вирусом SARS-CoV-2, продолжает оставаться крупнейшим глобальным вызовом для всего мира как по поиску методов лечения и профилактики, так разработке новых диагностических направлений. Одним из направлений серологической диагностики стала возмож...

A study on the reliability of an enzyme linked immunosorbent assay (ELISA) for the detection of typhoid fever, the ELISA-Ty test, was conducted, comprising 44 children suffering from bacteriologically confirmed typhoid fever based on the finding of a positive blood culture for Salmonella typhi, 44 children with fever caused by diseases other than typhoid fever based on the finding of negative c...

BACKGROUND Treatment with natalizumab, a humanized monoclonal antibody against alpha4beta1 integrin, is associated with an increase in lymphoid progenitor cells and B cells in peripheral blood. OBJECTIVE The objective of this study was to examine the impact of natalizumab therapy on serum levels of total IgG, IgA and IgM in patients with multiple sclerosis (MS). METHODS In two independent c...

Abstract Background Efficient monitoring and control of coronavirus disease 2019 (COVID-19) require access to diagnostic tests, serological testing is desirable. In the current study, antibodies were investigated in patients recently diagnosed with severe acute respiratory syndrome 2 (SARS-CoV-2) infection. Methods Cross-sectional data obtained from 245 whom SARS-CoV-2 infection had been confir...