Objective: The aim of this study was to determine the seroprevalence Toxoplasma gondii (T. gondii) in schizophrenia patients and reveal importance toxoplasmosis as a risk factor these patients. Method: conducted on 190 diagnosed with schizophrenia. Also included control group were 100 healthy individuals without Anti-T. immunoglobulin G (IgG) IgM antibody levels determined via enzyme-linked imm...

BACKGROUND Pan-IgG specific monoclonal antibodies (MAbs) are essential tools for assessment of humoral immunity, immune deficiency and gammopathy. In this study, four hybridoma clones producing MAbs with different specificities for human IgG isotypes were established. METHODS Splenocytes from Balb/c mice immunized with Fc fractions of human IgG were fused with SP2/0 myeloma cells. Hybridoma c...

An enzyme-linked immunosorbent assay (ELISA) for detection of immunoglobulin G (IgG) and IgM to cardiolipin, lecithin, and cholesterol (VDRL [Venereal Disease Research Laboratory] ELISA) is described. The specificity of the VDRL ELISA for IgG and IgM was 99.6 and 99.5%, respectively, with sera from 1,008 persons without syphilis. For a group of patients with false-positive results in traditiona...

IgG class antibodies reactive with myelin basic protein (MBP) were determined by enzyme-linked immunosorbent assay (ELISA) in serum and cerebrospinal fluid (CSF) of 37 patients with multiple sclerosis and a control group of 32 patients with tension headache or psychoneurosis. Using standardised amounts of IgG from CSF and serum in ELISA, significantly higher mean antibody levels were found in C...

In this study, we compared Coxiella burnetii IgG phase I, IgG phase II, and IgM phase II detection among a commercially available enzyme-linked immunosorbent assay (ELISA) (Virion/Serion), an indirect fluorescent antibody test (IFAT) (Focus Diagnostics), and a complement fixation test (CFT) (Virion/Serion). For this, we used a unique collection of acute- and convalescent-phase sera from 126 pat...

Estimates of the number of IgG molecules bound to normal platelets have ranged from several hundred to several tens of thousands. The lower estimates were generated from direct binding assays and stoichiometric assumptions. The higher values derive from competitive binding assays, in which platelet-associated IgG (PAIgG) is calculated from a standard curve using soluble IgG standards. Using a k...

BACKGROUND Brucellosis is a world-wide disease, which has a diverse clinical manifestation, and its diagnosis has to be proven by laboratory data. Serum agglutination test (SAT) is the most-widely used test for diagnosing brucellosis. The enzyme linked immunosorbent assay (ELISA) can also determine specific antibody classes against brucella. It is a sensitive, simple and rapid test, which could...

Introducción: Las pruebas inmunológicas son utilizadas como diagnóstico complementario en la vigilancia epidemiológica. Objetivos: Evaluar prueba inmunocromatográfica para detección de anticuerpos IgM e IgG contra el SARS-CoV-2, usando referencia ELISA IgM/IgG COVID-19. Metodología: El estudio realizado comparó inmunocromatográficas con IgM/IgG. También se efectuó estimación sensibilidad y espe...

background: pan-igg specific monoclonal antibodies (mabs) are essential tools for assessment of humoral immunity, immune deficiency and gammopathy. in this study, four hybridoma clones producing mabs with different specificities for human igg isotypes were established. methods: splenocytes from balb/c mice immunized with fc fractions of human igg were fused with sp2/0 myeloma cells. hybridoma c...