Herpes simplex virus type 1 (HSV-1) immediate-early (IE) proteins are required for the expression of viral early and late proteins. It has been hypothesized that host neuronal proteins regulate expression of HSV-1 IE genes that in turn control viral latency and reactivation. We investigated the ability of neuronal proteins in vivo to activate HSV-1 IE gene promoters (ICP0 and ICP27) and a late ...

Abstract Background Current FDA regulation defined acceptance level of 10 ng per dose residual DNA in drug products poses great challenges for accurate quantification DNA, particularly the used high doses or formulated complex matrixes. The purpose this study is to develop and qualify a qPCR method Chinese Hamster Ovary (CHO) antibody-based drugs quantitation human RNA-based drugs. Methods An 8...

Abstract Background Patients with injection drug use (IDU) have increased risk of developing infective endocarditis (IE). Previous studies reported recurrent IE, duration hospital stay, poor adherence and compliance as well higher mortality worse outcomes after surgery in the IDU-IE patient group. Further are needed to provide a basis for optimized care prevention readmissions this population. ...

Carbon nanotubes have been explored as heat-delivery vehicles for thermal ablation of tumors. To use single-walled carbon nanotubes (SWNT) as a "molecular heater" for hyperthermia therapy in cancer, stable dispersibility and smart-delivery potential will be needed, as well as lack of toxicity. This paper reports the preparation of a model complex comprising DNA-treated SWNT and anti-human IgG a...

We recently determined the genome sequence of the Coccolithoviridae strain Emiliania huxleyi virus 86 (EhV-86), a giant double-stranded DNA (dsDNA) algal virus from the family Phycodnaviridae that infects the marine coccolithophorid E. huxleyi. Here, we determine the phylogenetic relationship between EhV-86 and other large dsDNA viruses. Twenty-five core genes common to nuclear-cytoplasmic larg...

apres avoir presente l'origine, ie spectre de rayonnement et la totalite' de l'energie solaire arrivant sur la terre, nous citons tout d'abord les methodes et moyens d'utilisation de l'energie solaire dans ie passe(. nous presentons ensuite, les capteurs plans, concentration lineaire et ponctuelle; les moteurs et centrales solaires. puis nous discutons des generate...

The present study was carried out to characterize the cervical mucus (CM) collected when inseminating Holstein cows and to relate the secretion pattern with pregnancy. The mucus was collected from mid-cervix of 64 cows with spontaneus estrus (SE) and induced estrus (IE). The quantity, pattern and consistency of the mucus, pH, arborization patterns and the sperm motility were observed. The level...