New analytical techniques for multiparametric characterisation of individual cells are likely to reveal important information about the heterogeneity of immunological responses at the single-cell level. In this proof-of-principle study, laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) was applied to the problem of concurrently detecting 24 lineage and activation markers e...

.................................... ............................................................. vi CHAPTER 1. GENERAL INTRODUCTION ...................................................... 1 The History of Elemental Analysis ...................................................................... 1 ICP-MS ...............................................................................................

The aim of the study is to better understand where uranium deposits in mice kidneys. The spatial distribution of uranium was examined in the kidneys of C57BL/6 mice using laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). Mice were exposed to varying levels of uranyl nitrate in their drinking water. Calibration standards were developed to allow for semi-quantitative measur...

The Agilent 7500c Inductively Coupled Plasma Mass Spectrometer (ICP-MS) was used for the fast, routine, and reliable analysis of 23 trace elements in urine. The complete method validation for all elements is described, including the evaluation of short-term and long-term stability, the analysis of different reference materials and the discussion of precision and accuracy in internal and externa...

Since the fi rst generation of commercial ICP-MS appeared on the market in the 1980’s, the technique has been adopted for trace elemental analysis across a wide range of industries and applications. The measurement of high matrix samples typical of environmental, clinical, food, nuclear and geological applications, however, remains challenging. When high total dissolved solids (TDS) samples are...

Selenium (Se) is an important element in environmental and agricultural studies and in human health, as it is an essential trace nutrient but is also toxic in excess. The role of certain chemical forms of Se is also the subject of signifi cant research into cancer prevention. ICP-MS is the analytical method of choice for both total and speciated Se measurements, but Se is diffi cult to quantify...

High sensitivity and low background noise are critical for the detection of small nanoparticles by ICP-MS. The signal generated by the ionization of a nanoparticle decreases as the cube root of the diameter. This means detection of very small particles, such as 10 nm Au particles (NIST 8011), requires ICP-MS instrumentation with significantly higher sensitivity than is required for the more typ...

A dilution method using MnO(2) for the manganese nodule standard, JMn1, has been newly adopted to obtain accurate calibration lines. Moreover, an internal standard correction method was applied to correct changes in the sample introduction efficiency for the prime purpose of laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS) analysis. Using the dilution method and the cor...

Nanomaterials are increasingly used in food production and packaging, and validated methods for detection of nanoparticles (NPs) in foodstuffs need to be developed both for regulatory purposes and product development. Asymmetric flow field-flow fractionation with inductively coupled plasma mass spectrometric detection (AF(4)-ICP-MS) was applied for quantitative analysis of silver nanoparticles ...