The phosphonic analogue of L-phenylalanine, (/?)-(l-am ino-2-phenylethyl)phosphonic acid (A P E P ), inhibits buckwheat phenylalanine ammonia-lyase (PAL) com petitively with a K t value o f 1.5 |iM. The K, value for the (S)-enantiomer is 11.6 jj,M. The corresponding values for the enantiom ers of the phosphonous analogue are 35 and 205 |u.m , respectively. APEP inhibits the light-induced synthe...

Ultrasound-assisted extraction (UAE), using petroleum ether as the solvent, was systematically applied to extract main macamides and macaenes from Maca hypocotyls. Extraction yield was related with four variables, including ratio of solution to solid, extraction temperature, extraction time, and extraction power. On the basis of response surface methodology (RSM), the optimal conditions were de...

Influx and efflux of water and urea and electrolyte leakage are less for sunflower (Helianthus annuus) hypocotyl sections above lesions caused by Sclerotinia sclerotiorum than for those from healthy plants. Urea uptake by sections above lesions is reduced (celery, squash, and tomato) or unchanged (bean) in other hosts after Sclerotinia infection. Efflux of urea from sunflower hypocotyls is biph...

Plant peroxisomes play a pivotal role during postgerminative growth by breaking down fatty acids to provide fixed carbons for seedlings before the onset of photosynthesis. The enzyme composition of peroxisomes changes during the transition of the seedling from a heterotrophic to an autotrophic state; however, the mechanisms for the degradation of obsolete peroxisomal proteins remain elusive. On...

Methods Explants of normal porcine vaginal mucosa (full-thickness) were infected with biofilm-producing methicillinresistant S. aureus (MRSA) (3.2x10^5 CFU) for 2h – 48h. Following infection (2h), explants were treated with the active dressing (TegadermTM CHG) for 22-46h or left untreated (controls). Formation of MRSA biofilm was evaluated by scanning electron microscopy. Also, bacteria were en...

The regenerative potential in the root explants of in vivo grown Cattleya ’Almakee’ plants largely depend on the location of isolated explants from the main root and on the level&type of the growth regulators.Regeneration is affected by polarity all along the root axis of root.The regeneration is of basipetal gradient.The proximal explants responded to the presence of cytokinins(BAP/KN)in Mitra...

Plant peroxisomes play a pivotal role during postgerminative growth by breaking down fatty acids to provide fixed carbons for seedlings before the onset of photosynthesis. The enzyme composition of peroxisomes changes during the transition of the seedling from a heterotrophic to an autotrophic state; however, the mechanisms for the degradation of obsolete peroxisomal proteins remain elusive. On...

Effects of nitrogen (N) deficiency and sucrose (Suc) addition on regulation of anthocyanin biosynthesis and their relationship were investigated in this study. Radish sprouts subjected to N deficiency had 50% higher anthocyanin accumulation than when grown in Hoagland solution (a nutrient medium with all macronutrients). The contents of endogenous soluble sugars (Suc, fructose, and glucose) in ...

Mitochondria were isolated from nodules of cowpea (Vigna unguiculata (L). Walp.) and purified on a Percoll gradient. They were only slightly contaminated by bacteroids (an average of 3.5%), and had low lipoxygenase activity. Compared to mitochondria from hypocotyls the nodule mitochondria had similar O(2) uptake rates and respiratory control ratios. The ADP/O ratios for both preparations were 1...

Cereus peruvianus (Cactaceae) is an important medical plant. The study was carried out on callus induction of Cereus jamacaru f. monstrosus and Cereus hildmannianus fma monstrosa (Cereus peruvianus). Apical and lateral explants were cultured on Murashige and Skoog media with factorial combinations of the auxins indole-3acetic acid (NAA), 2, 4-dichlorophenoxyacetic acid (2,4-D) and N-(2-furanyl-...