Zygomycetes is a class of fungi living worldwide as saprobes, as part of mycorrhizae, and as parasites. Humans have used some zygomycetes for centuries in the production of traditional foods, e.g. Indonesian tempe. In the present thesis, the experimental focus was on two zygomycetes strains, Mucor indicus CCUG 22424 and Rhizopus sp. IT. One of the distinguishing features of M. indicus is its di...

The hydrolysis of sodium borohydride as a source of hydrogen gas is studied at different mole ratios of H+ to NaBH4. The sulfonated porous carbon (SPC) is used as a source of hydrogen ion and catalyst. It is found that the sulfonated porous carbon exhibits high activity for the hydrolysis of NaBH4 to generate hydrogen gas at room temperature in comparison to Amberlyst-15 and Nafion-NR50. The ki...

The repair of DNA double-strand breaks (DSBs) is essential to maintaining the integrity of the genome, and organisms have evolved a conserved mechanism to facilitate their repair. In eukaryotes, archaea, and some bacteriophage, a complex made up of Mre11 and Rad50 (MR complex), which are a nuclease and ATPase, respectively, is involved in the initial processing of DSBs. Rad50 is a member of the...

BACKGROUND In the hydrolysis of lignocellulosic materials, thermostable enzymes decrease the amount of enzyme needed due to higher specific activity and elongate the hydrolysis time due to improved stability. For cost-efficient use of enzymes in large-scale industrial applications, high-level expression of enzymes in recombinant hosts is usually a prerequisite. The main aim of the present study...

A comprehensive picture of structural changes of cellulosic biomass during enzymatic hydrolysis is essential for a better understanding of enzymatic actions and development of more efficient enzymes. In this study, a suite of analytical techniques including sum frequency generation (SFG) spectroscopy, infrared (IR) spectroscopy, x-ray diffraction (XRD), and x-ray photoelectron spectroscopy (XPS...

Freeze dried protein powders (Fresh minced meat, FMM and Hot water dip, HWD) from tilapia (Oreochromis niloticus) were hydrolyzed by Alcalase 2.4 L (Alc), Flavourzyme (Flav) and Neutrase (Neut), and investigated for antioxidant activity and their functional properties. FMM and HWD hydrolysed by Alc, exhibiting superior antioxidant activity, had estimated degrees of hydrolysis (DH) of 23.40% and...

the hydrolysis of cadmium nitrate was studied spectrophotometrically at different ionic strengthsof sodium perchlorate in a wide ph range, 1-12, and at 25 c. least squares calculations areconsistent with the formation of m(oh)+, m(oh)2, m(oh)3- and m(oh)42- species, which m iscd2+. the dependency on ionic strength in the background electrolyte solutions was taken intoaccount by using a debye-hu...

Due to the presence of dyes and additives, textile recycling is challenging, therefore majority waste downcycled low-value products, incinerated or landfilled. In this study, a continuous depolymerization post-consumer polyester textiles was conducted by alkaline hydrolysis. The degree assessed found be 94 %. After recovering analyzing terephthalic acid ethylene glycol monomers were successfull...

INTRODUCTION Poly(vinyl alcohol) (PVA) is a highly hydrophilic, nontoxic and biocompatible semicrystalline polymer with excellent properties such as strength, water solubility, gas permeability and thermal characteristics. PVA is available in a variety of degrees of hydrolysis (DH) because it is derived from the hydrolysis (or alcoholysis) of poly(vinyl acetate) (PVAc).1–3 The properties of PVA...

Dietary proteins are no longer just nutritional ingredients in our food. During hydrolysis, some of the released peptides may possess properties that favor health human body. In study enzymatic hydrolysis lupin was performed using papain. Three enzyme-to-substrate ratios were set for three different duration times. The SDS-PAGE samples performed. Each hydrolysate studied degree (DH), acetylchol...