ABS TRACT Introduction: Cutaneous fillings are a common procedure in today’s dermatology, with the majority being carried out with hyaluronic acid isolated or combined with other substances. Objective: To study the effects of adding hyaluronic acid and polyethylene glycol to cultures of human dermal fibroblasts. Methods: The study evaluated: cell proliferation and production of type 1 collagen ...

UNLABELLED The vibration of the vocal fold lamina propria is an important factor involved in vocal production and aging may change the amount of hyaluronic acid in the vocal fold leading to dysphonia. AIMS This study compares the concentration of hyaluronic acid in vocal folds of aged and young female rats. STUDY DESIGN experimental. MATERIALS AND METHODS We used the vocal cords of 13 fem...

In tissue engineering, an ideal scaffold attracts and supports cells thus providing them with the necessary mechanical support and architecture as they reconstruct new tissue in vitro and in vivo. This manuscript details a novel matrix derived from decellularized Wharton's jelly (WJ) obtained from human umbilical cord for use as a scaffold for tissue engineering application. This decellularized...

It has been demonstrated in vitro that low concentrations of ascorbic acid rapidly depolymerize purified hyaluronic acid as judged from viscosity measurements (Skanse and Sundblad, 1943; Pigman, Rizvi, and Holley, 1961). Abrams and Sandson (1964) reported that the hyaluronic acid in rheumatoid synovial fluid is resistant to depolymerization by ascorbic acid in vitro. Serum proteins have been sh...

We propose a culture-free approach to osteochondral repair with minced autologous cartilage fragments loaded onto a scaffold composed of a hyaluronic acid (HA)-derived membrane, platelet-rich fibrin matrix (PRFM) and fibrin glue. The aim of the study was to demonstrate in vitro the outgrowth of chondrocytes from cartilage fragments onto this scaffold and, in vivo, the formation of functional re...

This work follows from that of Blumberg & Ogston (1957). They showed that papain is a specific agent for degrading the protein part of the hyaluronic acid complex isolated from ox synovial fluid by filtration (Ogston & Stanier, 1950), and that when this protein is degraded marked changes in the non-Newtonian viscosity occur. This is associated with a marked decrease in the molecular weight and ...

To develop and optimize new scaffold materials for tissue engineering applications, it is important to understand how changes to the scaffold affect the cells that will interact with that scaffold. In this study, we used a hyaluronic acid- (HA-) based hydrogel as a synthetic extracellular matrix, containing modified HA (CMHA-S), modified gelatin (Gtn-S), and a crosslinker (PEGda). By varying th...

The effective and sustained delivery of DNA locally could increase the applicability of gene therapy in tissue regeneration and therapeutic angiogenesis. One promising approach is to use porous hydrogel scaffolds to encapsulate and deliver nucleotides in the form of nanoparticles to the affected sites. We have designed and characterized microporous (μ-pore) hyaluronic acid hydrogels which allow...

Cartilage defects resulting from traumatic injury or degenerative diseases have very limited spontaneous healing ability. Recent progress in tissue engineering and local therapeutic gene delivery systems has led to promising new strategies for successful regeneration of hyaline cartilage. In the present study, tissue engineering and local therapeutic gene delivery systems are combined with the ...

Autologous chondrocyte transplantation (ACT) has been shown, in long-term follow-up studies, to be a promising treatment for the repair of isolated cartilage lesions. The method is based on an implantation of in vitro expanded chondrocytes originating from a small cartilage biopsy harvested from a non-weight-bearing area within the joint. In patients with osteoarthritis (OA), there is a need fo...