Abstract In an attempt to develop new anti‐inflammatory agents which act by co‐release of carbon monoxide (CO) and a specialized pro‐resolving mediator, we designed conjugates lipoxin A 4 analogue acyloxycyclohexadiene‐Fe(CO) 3 complex as esterase‐triggered CO‐releasing molecule (ET‐CORM). After adjustment the protecting group strategy, two such compounds were successfully prepared total synthe...

Peripheral polymorphonuclear leukocytes (PMNL) in hemodialysis patients (HD) are primed, continually releasing and exposing the vascular endothelium to soluble factors such as reactive oxygen species (ROS) and inflammatory mediators. To mimic the close proximity between PMNL and the endothelial monolayer, and to monitor and characterize the influence of soluble mediators released from PMNL, we ...

The objective of this research was to test the in vitro anti-angiogenic activity of auraptene, a coumarin from citrus peel oil. We tested the ability of auraptene to inhibit in vitro angiogenesis using vascular endothelial growth factor (VEGF)-induced human umbilical endothelial cells (HUVEC) growth stimulation. Auraptene was applied at concentrations between 0 μM and 1μM to HUVEC in the presen...

Lysine-plasminogen (Lys-PLG), the plasmin-modified form of native glutamic acid-plasminogen (Glu-PLG), displays enhanced binding affinity for fibrin and also enhanced activation by urokinase and tissue plasminogen activator. We previously demonstrated high-affinity, specific, and functional binding of Glu-PLG as well as tissue plasminogen activator to cultured human umbilical vein endothelial c...

Staphylococcus aureus is isolated from a substantial number of patients with infective endocarditis who are not known to have predisposing heart abnormalities. It has been suggested that the infection is initiated by the direct binding of S. aureus to human vascular endothelium. To determine the mutual response of the endothelial cells and the bacteria, we studied the interaction between S. aur...

Extracellular matrix (ECM) serves a key role in cell migration, attachment, and cell development. Here we report that ECM derived from human umbilical vein endothelial cells (HUVEC) promoted osteogenic differentiation of human bone marrow mesenchymal stem cells (hMSC). We first produced an HUVEC-derived ECM on a three-dimensional (3D) beta-tricalcium phosphate (β-TCP) scaffold by HUVEC seeding,...

Restricted by insufficient waterproofness and stretching resistance, hydrogel alone is seldomly applied in bladder wall regeneration. A tri-layer scaffold comprising acellular matrix graft-alginate di-aldehyde-gelatin hydrogel-silk mesh (BAMG-HS) was designed to encapsulate human adipose stem cells (ASCs)-derived extracellular vesicles (EVs), aiming facilitate The proangiogenic composite biomat...

Poly(ether imide) (PEI) is being explored as potential biomaterial for cardiovascular applications. Different studies showed that human umbilical venous endothelial cells (HUVEC) are able to adhere and proliferate on PEI membranes (R(q) = 13.20 ± 1.58 nm). A recently published study revealed evidence for much lower platelet adhesion on very smooth PEI-films (R(q) = 2.37 ± 1.40 nm). Therefore, w...

The phenotype of endothelial cells (ECs) is specific to the vascular bed from which they originate. To examine how mechanical forces alter the phenotype of different ECs, we compared the effects of cyclic strain and motion control on reactive oxygen species (ROS) production and metabolism and cell adhesion molecule expression in human umbilical vein endothelial cells (HUVEC) vs. human aortic en...