despite many advances in the treatment of breast cancer, it is still the second most common cause of death in women in the united states. it has been shown that inflammation plays a major role in the treatment of these cancers and inflammatory factors enhance tumor growth, invasion, metastasis, and vascularization. in this study, we would like to analyze peripheral blood neutrophil-lymphocyte r...

The tissue-specific homing of lymphocytes is directed by specialized high endothelial venules (HEV). At least three functionally independent lymphocyte/HEV recognition systems exist, controlling the extravasation of circulating lymphocytes into peripheral lymph nodes, mucosal lymphoid tissues (Peyer's patches or appendix), and the synovium of inflamed joints. We report here that antibodies capa...

Insulin interactions with human lymphocytes in established cultures and with isolated peripheral lymphocytes have been studied using 1251-insulin. Receptors in circulating lymphocytes are indistinguishable from those found in cultured cells, and the lymphocyte receptors show striking similarities to those structures described in fat cell and liver preparations. In lymphocytes, the ability of in...

NKG2D is a critical activatory molecule expressed on cytotoxic cells such as NK cells, γδ T cells and subsets of αβ T cells [1]. Ligation of NKG2D acts to trigger cytotoxicity of NK cells and acts as a co-stimulatory molecule on T cells. The ligands that bind to NKG2D, known as NKG2DL, are grouped into two families and comprise eight different proteins termed MIC-A/B and ULBP1-6 (Figure 1a). Im...

Induction of thymus-dependent lymphocyte (T cell) differentiation was performed in vitro with thymic factors as inducers. T cell precursors from human bone marrow first expressed surface differentiation antigens and then acquired the capacity to form rosettes with sheep erythrocytes. The latter marker could not be induced when cells with differentiation antigens had been eliminated. The prolife...

Human peripheral blood lymphocytes are stained with the metachromatic dye acridine orange and the fluorescence of individual cells is measured by flow cytometry. The relative content of stainable RNA per cell is estimated by comparison with RNase-treated cells. Non-T and T lymphocytes have different mean quantities of RNA per cell, and these classes exhibit different distributions of RNA conten...

The role of specific cell-mediated immune responses in human cytomegalovirus (CMV) infections was studied by an in vitro lymphocyte transformation test, using a whole blood culture. CMV-induced in vitro lymphocyte proliferation was mainly dependent on the presence of sensitized T-cells. Three of five seronegative patients with B-cell deficiency showed positive lymphocyte responses to CMV antige...