Oncogenic mutations in the PI3K/AKT pathway are present in nearly half of human tumors. Nonetheless, inhibitory compounds of the pathway often induce pathway rebound and tumor resistance. We find that lung squamous cell carcinoma (SQCC), which accounts for ~20% of lung cancer, exhibits increased expression of the PI3K subunit PIK3R2, which is at low expression levels in normal tissues. We teste...

Tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) selectively induces apoptosis in cancer cells but not in normal cells. However, studies have indicated that more than half of human tumors exhibit TRAIL resistance. Although the mechanism of TRAIL resistance is not understood, it represents a barrier to any TRAIL-mediated gene therapy approach. In addition, no correlation bet...

Using a magnet-assisted subtraction technique, 17 complementary DNA (eDNA) clones were isOlatedthat were expressed in the normal lung but were decreased or lost In the corresponding tumor tissue of a non small cell lung carcinoma patlent@The luck of expression of six magnet assisted subtraction technique eDNA clones in three additional non-small cell lung cardnoma cases indicates their possible...

Lung cancer is the leading cause of cancer-related deaths worldwide; non-small cell lung cancer (NSCLC), which includes squamous cell lung carcinoma, adenocarcinoma, large-cell lung carcinoma, accounts for more than 80% lung malignancy (Jemal et al., 2007). Despite recent advances in multimodality therapies, the prognosis for most patients with NSCLC is still unsatisfactory (Pfister et al., 200...

Fourier Transformed Infrared Spectroscopy (FTIR) has extensively been used for biological applications. Cisplatin is one the most useful antineoplastic chemotherapy drugs for a variety of different human cancers. One of the clinical problems in its application, which would consequently affect the therapeutic outcome of its application, is the occurrence of resistance to this agent. In this proj...

A new clerodane diterpene, Caseargrewiin M (1), and one known compound (2) were isolated from fresh ripe fruits of Casearia grewiifolia. The structures of all isolated compounds were evaluated on the basic 1D (H and C) and 2D NMR (COSY, HSQC, HMBC and NOESY) spectral data. All compounds were examined cytotoxicity with five human cancer cell lines, Hep-G2 (hepatocarcinoma), SW620 (colon adenocar...

The purpose of this study was to determine whether sustained local production of murine IFN-beta (mIFN-beta) could inhibit the tumorigenicity and metastasis of human and murine tumor cells implanted into nude mice. Human melanoma cells (A375SM), renal carcinoma cells (SN12PM6), and colon carcinoma cells (KM12SM) were transfected with mIFN-beta or a control neomycin resistance vector. All cell l...

A mouse monoclonal antibody, WI-MN-1, was raised against G-361 melanoma cell line. Reactivity of this antibody was characterized by indirect immunofluorescence against 22 cell lines and normal and neoplastic human tissues. Positive reactions were seen against three melanoma cell lines (G-361, HT-144, and MeWo). It also reacted against an epidermoid carcinoma cell line (Hep-2) and amnion cells (...