Thrombospondin, a major glycoprotein released from alpha granules of thrombin-stimulated platelets, is a disulfide-bonded trimer of 160-kilodalton subunits. Cultured human foreskin and fetal lung fibroblasts secreted thrombospondin (determined by enzyme-linked immunosorbent assay) into the culture medium in a time-dependent manner (15.7 and 5.8 micrograms per 10(6) cells per 24 hr, respectively...

plant-derived natural products are known to have cancer chemo-preventive and chemo-therapeutic properties. plant extracts or their active constituents are used as folk medicine in traditional therapies by 80% of the world population. the aim of the present study was to determine the anti-proliferative potential of fumaria vaillantii extracts on three different cancer cell lines including malign...

Infectious bursal disease (IBD), also known as Gumboro disease, is a globally well-known disease with a significant socio-economic effect. For control of IBD, several commercial egg- and cell-based vaccines are prepared. The cell-based IBD vaccines are significantly cost-effective; however, it is essential to confirm their safety and efficacy. The main cell line used to product the cell-based I...

background: although the incidence of gastric cancer is declining during the last half century, this cancer still is the second morbid cancer in the world after lung cancer. the incidence of gastric cancer is 26 per 100,000 in iran. this study evaluated the effect of alpinia galangal on ags cells (human gastric adenocarcinoma epithelial cell line) and l929 cells (as a standard cell line origina...

Two consecutive prefractionated fractions of the Australian marine sponge extract, Pipestela candelabra, were identified to be selectively active on the human prostate cancer cells (PC3) compared to the human neonatal foreskin fibroblast non-cancer cells (NFF). Twelve secondary metabolites were isolated in which four compounds are new small peptides. Their structures were characterized by spect...

Two distinct types of cDNA clones encoding for the pyruvate dehydrogenase (PDH) E1 beta subunit were isolated from a human liver lambda gt11 cDNA library and characterized. These cDNA clones have identical nucleotide sequences for PDH E1 beta protein coding region but differ in their lengths and in the sequences of their 3'-untranslated regions. The smaller cDNA had an unusual polyadenylation s...

Dihydroactinidiolide (1) and a mixture of sterols [campesterol (2), stigmasterol (3) and beta-sitosterol (4)], together with the previously isolated individual compounds beta-sitosterol (4), 2,4-di-tert-butylphenol (5), alpha-tocopherol (6), phytol (7) were isolated from the active ethyl acetate fraction of Pereskia bleo (Kunth) DC. (Cactaceae) leaves. Cytotoxic activities of the above mentione...

Achillea eriophora D.C. (Asteraceae), an endemic species in Iran, is used extensively in Iranian folk medicine to treat various ailments, including gastrointestinal disorders, skin inflammations and wound healing. The present study was aimed to investigate the effects of the methanolic extract of  A. eriophora leaves on the stimulation of human fibroblast cell proliferation and migration as two ...