Gamma-glutamyl carboxylase (GGCX) gene mutation causes GGCX syndrome (OMIM: 137167), which is characterized by pseudoxanthoma elasticum (PXE)-like symptoms and coagulation impairment. Here, we present a 55-year-old male with a novel homozygous deletion mutation, c.2,221delT, p.S741LfsX100, in the GGCX gene. Histopathological examination revealed calcium deposits in elastic fibers and vessel wal...

Clinical evidence suggests that skin is responsive to GH status in vivo. We sought to demonstrate the presence of GH receptors in human skin and in cultured skin fibroblasts using the techniques of immunohistochemistry and northern blotting. Human foreskin was obtained at surgery for preparation of sections and primary fibroblast cultures. Skin sections and fibroblast monolayers were immunostai...

Fibroblasts and their activated phenotype, myofibroblasts, are the primary cell types involved in the contraction associated with dermal wound healing. Recent experimental evidence indicates that the transformation from fibroblasts to myofibroblasts involves two distinct processes: The cells are stimulated to change phenotype by the combined actions of transforming growth factor β (TGFβ) and me...

Little is known about miRNA decay, especially in skin wound healing. Here, by small RNA sequencing human and tissues, we identified 12 miRNAs that 5P:3P strand ratios significantly changed. To rule out the effects caused cellular composition, performed CD45- epidermal cells (mainly keratinocytes) CD90+ dermal (fibroblasts) isolated from wounds. Our results showed ratio of miR-X changed both cel...

Dermal fibroblasts are transcriptomically heterogeneous. In spite of numerous single-cell RNA sequencing (scRNAseq) studies dedicated to decipher fibroblast heterogeneity, previous approaches lack sufficient insight and do not establish a bridge between mouse human data. We aimed obtain comprehensive map mouseskin from pre-existing To this end, 30 independent scRNAseq datasets (22 including der...

miRNAs as one of the potential therapeutic agents have been recently considered for cancer treatment. AS1411 (aptNCL) is a DNA aptamer specifically binding to nucleolin protein on the cancer cell surface with antiproliferative effect. The aim of the study was to develop a conjugate consisted of aptNCL (as targeted delivery of therapeutic agent) and miRNA let-7d (as a tumor suppressor) using two...

Suppression of collagen synthesis is a major therapeutic goal in the treatment of fibrotic disorders. We show here that alpha-melanocyte-stimulating hormone (alpha-MSH), a neuropeptide well known for its pigment-inducing capacity, modulates collagen synthesis and deposition. Alpha-MSH in vitro suppresses the synthesis of collagen types I, III, and V and down-regulates the secretion of procollag...

Adiponectin is one of the most abundant adipokines from the subcutaneous fat, and regulates multiple activities through endocrine, paracrine, or autocrine mechanisms. However, its expression in adipogenic induced fibroblasts, and the potential role in photoaging has not been determined. Here, human dermal fibroblasts, Hs68, were presented as a cell model of dermal lipogenesis through stimulatio...

PURPOSE The level of constitutive plasminogen activator inhibitor type-1 (PAI-1) expression in cultured human orbital fibroblasts is considerably lower than that found in dermal fibroblasts. This divergence in PAI-1 expression implies differences in the pericellular proteolytic environment and, therefore, in the turnover of extracellular matrix. In this article, the authors examine the effect o...

Although infrared radiation (IRR) is ubiquitous in the terrestrial milieu, its effects on human skin have until now been largely ignored. Recent studies suggest an important role for infrared A (IRA) radiation (760-1440 nm) in dermal inflammation, photoaging, and photocarcinogenesis. In this issue, Calles et al. identify and analyze the IRA-induced transcriptome in human dermal fibroblasts. The...