The National Toxicology Program Interagency Center for the Evaluation of Alternative Toxicological Methods (NICEATM) provides validation support for US Federal agencies and the US Tox21 interagency consortium, an interagency collaboration that is using high throughput screening (HTS) and other advanced approaches to better understand and predict chemical hazards to humans and the environment. T...

Cryptosporidium is an important enteric pathogen that infects a wide range of humans and animals. Rapid and reliable detection and characterisation methods are essential for understanding the transmission dynamics of the parasite. Sanger sequencing, and high-throughput sequencing (HTS) on an Ion Torrent platform, were compared with each other for their sensitivity and accuracy in detecting and ...

PURPOSE Macrophage migration inhibitory factor (MIF) may serve as a general marker for systemic inflammation in septic and nonseptic acute critical illness. Additionally, our previous experiment has demonstrated that immunosuppressant Prostaglandin E(2) (PGE(2)) lowered MIF levels and inhibited T-cells proliferation when compared to control levels. The addition of hypertonic saline (HTS) increa...

Guanine-rich oligonucleotides are able to form four-stranded G-quadruplex structures by associating stacks of G-quartets which are stabilized by Hoogsteen hydrogen bonding. Recently, G-quadruplex structures have drawn lots of attention in areas such as biology, medicinal chemistry, supramolecular chemistry and nanotechnology. G-quadruplex structures display great conformational diversity due to...

The primary cause of poor outcome following allogeneic hematopoietic cell transplantation (HCT) for chronic lymphocytic leukemia (CLL) is disease recurrence. Detection of increasing minimal residual disease (MRD) following HCT may permit early intervention to prevent clinical relapse; however, MRD quantification remains an uncommon diagnostic test because of logistical and financial barriers to...

Combining high throughput sequencing with stable isotope probing (HTS-SIP) is a powerful method for mapping in situ metabolic processes to thousands of microbial taxa. However, accurately mapping metabolic processes to taxa is complex and challenging. Multiple HTS-SIP data analysis methods have been developed, including high-resolution stable isotope probing (HR-SIP), multi-window high-resoluti...

A new hybrid tabu search (HTS) method for solving assembly line balancing problems is proposed in this paper. The tabu search (TS) method is combined with the genetic algorithm (GA) to identify and provide solutions for assembly line balancing problems. With the proposed HTS method, the TS method can well address the number of tasks assigned for each workstation, while the GA can also assign th...

II. THEORY AND SIMULATION Abstract— A novel THz device concept called as CW HTS (high temperature superconductive) photomixer/antenna is proposed. Optical heterodyne photomixing in the DC-biased HTS strip has been employed to create THz signal, and the size of the strip on the grounded dielectric substrate is designed to have an efficient broadside radiation. Incorporating the HTS microstrip co...

Aptamers are identified through an iterative process of evolutionary selection starting from a random pool containing billions of sequences. Simultaneously to the amplification of high-affinity candidates, the diversity in the pool is exponentially reduced after several rounds of in vitro selection. Until now, cloning and Sanger sequencing of about 100 sequences was usually used to identify the...

We discuss an Haimonic Balance algorithm to analyze High Temperature Superconducting Circuits (HTS) subject to wideband signals, such as t h e ones used in C D M A wireless systems. Undersampling is used to discretize and down-convert t h e bandpass signal resulting from the weak HTS nonlinearities, and this has to b e taken into account when performing t imedomain calculations of the nonlinear...