Oyekan, A. O., K. McAward, J. Conetta, L. Rosenfeld, and John C. McGiff. Endothelin-1 and CYP450 arachidonate metabolites interact to promote tissue injury in DOCAsalt hypertension. Am. J. Physiol. 276 (Regulatory Integrative Comp. Physiol. 45): R766–R775, 1999.—Inhibition of cytochrome P-450 (CYP450) enzymes with cobalt chloride (CoCl2) prevented hypertension, organ hypertrophy, and renal inju...

Objective(s):The limited homing potential of bone-marrow-derived mesenchymal stem cells (BM-MSC) is the key obstacle in MSC-based therapy. It is believed that chemokines and chemokine receptor interactions play key roles in cellular processes associated with migration. Meanwhile, MSCs express a low level of distinct chemokine receptors and they even lose these receptors on their surface after a...

Abbreviations: LLOQ: Lower Limit of Quantification; QC: Quality Control; LQC: Low QC; MQC: Middle QC; HQC: High QC; SPE: Solid Phase Extraction; MRM: Multiple Reaction Monitoring; CV: Coefficient of Variation; RE: Relative Error; QqTOF: Quadrupole-QuadrupoleTime-Of-Flight; FDA: Food and Drug Administration; EMA: European Medicines Agency; XIC: Extracted-Ion Chromatogram; AUC: Area under the Cur...

We evaluated the contribution of cytochrome P-450 (CYP450)-dependent arachidonic acid (AA) metabolites and prostanoids to the renal hemodynamic and tubular effects of endothelin-1 (ET-1) in anesthetized rats. Either ET-1 (0.3, 1.0, and 3 pmol ⋅ kg-1 ⋅ min-1) or vehicle was infused intravenously during two to three 30-min clearance experimental periods. Only high-dose ET-1 increased mean arteria...

Cucumis melo L. is one of the most economically important horticultural crops worldwide. However,   low plant regeneration frequency of this plant during genetic transformation is  the  major hurdle for  applying biotechnological approaches. Hence, this study aimed to evaluate the effect of 6-benzyladenine (BA), cefotaxime (CTX), kanamycin (KAN), and indole-3-acetic acid (IAA) on the regenerati...

BACKGROUND AND OBJECTIVES We recently reported that hypoxia-mimetic agents cobalt chloride (CoCl2 CoCl2 ) and desferrioxamine (DFO) could induce differentiation of acute myeloid leukemic (AML) cells. Here, we investigate whether these two agents influence the in vitro differentiation-inducing effect of arsenic trioxide (As2O3) on AML cells, an effective drug for the treatment of acute promyeloc...

BACKGROUND Perioperative hypoxia may induce microglial inflammation and apoptosis, resulting in brain injury. The neuroprotective effect of propofol against hypoxia has been reported, but the underlying mechanisms are far from clear. In this study, we explored whether and how propofol could attenuate microglia BV2 cells from CoCl2-induced hypoxic injury. METHODS Mouse microglia BV2 cells were...

It is well known that cobalt chloride (CoCl2) can enhance the stability of hypoxia-inducible factor (HIF)-1α. The aim of this study is to detect the effect of CoCl2 on the hypoxia tolerance of mice which were repeatedly exposed to autoprogressive hypoxia. Balb/c mice were randomly divided into groups of chemical pretreatment and normal saline (NS), respectively injected with CoCl2 and NS 3 h be...