The fingerprint profiles of methanol extract and ethyl acetate sub fraction of Azadirachta indica leaves were conducted using preliminary phytochemical screening and high performance thin layer chromatography (HPTLC) analysis. Preliminary phytochemical screening was performed using various biochemical tests. Alkaloid, flavonoid, tannin, saponin and phenol were present in both the extract/subfra...

The host immune system is the first line of host defense, consisting mainly of innate and adaptive immunity. Immunity must be maintained, orchestrated, and harmonized, since overactivation of immune responses can lead to inflammation and autoimmune diseases, while immune deficiency can lead to infectious diseases. We investigated the regulation of innate and adaptive immune cell activation by A...

To establish a method for determining ursolic acid content in Patrinia scabiosifolia Fisch. ex Trevir.; and to investigate the inhibitory effect of Patrinia scabiosifolia Fisch. ex Trevir. extract on in vitro cultured human cervical carcinoma Hela cells. HPLC with Agilent XBP-C18 column (4.6 mm × 250 mm, 5 μm) was used. Mobile phase was acetonitrile-methanol-0.5% ammonium acetate solution (68:1...

Ursolic acid (UA), a pentacyclic triterpenoid, is known to exert antitumor activity in breast, lung, liver and colon cancers. Nonetheless, the underlying mechanism of ursolic acid in prostate cancer cells still remains unclear. In the present study, we report the chemotherapeutic effects of ursolic acid as assessed using in vitro and in vivo models. Treatment of human prostate cancer cells (LNC...

1 Regional Innovation Center for Dental Science and Engineering, Chosun University, 309 Pilmun-daero, Dong-gu, Gwangju, 501-759, Korea 2 Department of Biosystems Machinery Engineering, Chungnam National University, 99 Daehak-ro, Yuseong-gu, Daejeon, 305-764, Korea 3 Department of Crop Science, Chungnam National University, 99 Daehak-ro, Yuseong-gu, Daejeon, 305-764, Korea * Corresponding author...

Mentha piperita and Citrus sinensis was an aromatic plants well known for its fragrance and distinct aroma. In this prospective study to evaluate the chromatogram detection of ethanol extract of Mentha piperita and Citrus sinensis with standard flavonoid marker eriocitrin by HPTLC technique. HPTLC chromatogram was developed in ethanolic extract of Mentha piperita, Citrus sinensis and standard f...

Nano-sized polymeric amphiphilic micelle was prepared using the hydrophilic polysaccharide, oligo-chitosan, and hydrophobic side chains, ursolic acid which assists the skin penetration, because the amphiphilic polysaccharide nanoparticles have been widely investigated as the carriers for active agents such as small molecules, proteins, peptides, and nucleic acids due to many advantages in prote...

The objective of the present work was to develop a simple, specific, and fast high-performance thin-layer chromatographic (HPTLC) method identify quantify cordifolioside A, 20-β-hydroxyecdysone columbin with HPTLC‒electrospray ionization‒tandem mass spectrometry (ESI‒MS/MS) for characterization in Tinospora cordifolia stem extracts. Chromatographic development performed using HPTLC aluminum pla...