Filtration is a common method of sample preparation in dissolution testing, prior to HPLC injection. The purpose of sample filtration is to remove non-dissolved solids prior to HPLC injection. Non-dissolved solids interfere with the resulting chromatography by continuing to dissolve throughout the period of the analysis and also by plugging the HPLC column. It has already been shown that sample...

Mycolic acids extracted from saponified mycobacterial cells were examined as p-bromophenacyl esters by high-performance liquid chromatography (HPLC). Standard HPLC patterns were developed for species of Mycobacterium by examination of strains from culture collections and other well-characterized isolates. Relative retention times of peaks and peak height comparisons were used to develop a diffe...

Nicotinamide, one kind of Vitamin B, plays an important role for humans. To ensure quality control of nicotinamide in infant formula, a Certified Reference Material for nicotinamide in infant formula is being prepared in China for the first time. Studying on the procedure of sample preparation and HPLC analysis with different detectors like UV and MS, HPLC-UV and HPLC-IDMS are selected as the v...

Objective: The present work was undertaken with the aim to develop and validate a rapid and consistent RP-HPLC method in which the peaks will be appear with short period of time as per ICH Guidelines. Methods: The proposed method was simple, fast, accurate and precise method for the Quantification of drug in the dosage form, bulk drug as well as for routine analysis in Quality control. Reversed...

HPLC analysis is important for determination of total level on DNA methylation in plants. It can be used to help characterise epigenetic changes during growth, development and stress. HPLC methods have been optimised for mammalian and microbial DNA, but not for plants. This article examines several important factors in the HPLC analysis of plant DNA methylation including extraction and purifica...

We here report a reversed-phase HPLC method for the determination of free cortisol in human urine, using methylprednisolone as the internal standard. Before chromatography, samples were extracted with a C18 solid-phase extraction column and the steroids were separated on a LiChrospher 100 C18 column with a mobile phase of methanol/acetonitrile/water (43/3/54 by vol). Linearity, precision, and a...

In this study, an established HPLC method for USP Phenobarbital was sequentially and quickly improved to a high throughput method using Rapid Resolution (RR) and Rapid Resolution High Throughput (RRHT) techniques. New RRHT column technology is an especially powerful tool for improving lab productivity by reducing HPLC analysis time dramatically. Starting with a proven rugged method, conversion ...

The use of the RP-HPLC official method of the International Honey Commission (IHC) for the determination of 5-hydroxymethylfurfural (HMF) in strawberry tree honey (Arbutus unedo, a typical Sardinian honey) has brought to light a specific and heavy chromatographic interference that prevents accurate quantification. The interference has been identified as homogentisic acid (HA), i.e. the marker o...

The aim of the present study was to develop and validate an inexpensive and simple high performance liquid chromatographic (HPLC) method for the determination of colistin sulfate. Separation of colistin sulfate was achieved on a ZORBAX Eclipse XDB-C18 column using UV detection at λ=215 nm. The mobile phase was 30 mM sulfate buffer (pH 2.5): An excellent linearity (r=0.998) was found in the conc...

A rapid, simple and specific reversed-phase HPLC method has been developed for analysis of karanjin in Pongamia pinnata Linn. leaves. HPLC analysis was performed on a C(18) column using an 85:13.5:1.5 (v/v) mixtures of methanol, water and acetic acid as isocratic mobile phase at a flow rate of 1 ml/min. UV detection was at 300 nm. The method was validated for accuracy, precision, linearity, spe...