Using solid-phase extraction columns and "high-performance" liquid-chromatographic (HPLC) analysis, we could determine cyclosporin A and nine of its metabolites in blood, bile, and urine. To facilitate calculations of concentrations of cyclosporin A and its metabolites from the chromatograms, we used cyclosporin D as internal standard. For the HPLC analysis we used two sequential 250-mm analyti...

BACKGROUND Indoor residual spraying (IRS) of DDT is used to control visceral leishmaniasis (VL) in India. However, the quality of spraying is severely compromised by a lack of affordable field assays to monitor target doses of insecticide. Our aim was to develop a simple DDT insecticide quantification kit (IQK) for monitoring DDT levels in an operational setting. METHODOLOGY/ PRINCIPLE FINDIN...

A number of analytical methods have been established to quantify methylphenidate (MPH). However, to date no HPLC methods are applicable to human pharmacokinetic studies without the use of mass spectrometry (MS) detection. We developed a sensitive and reliable HPLC-fluorescence method for the determination of MPH in human plasma using 4-(4,5-diphenyl-1H-imidazol-2-yl) benzoyl chloride (DIB-Cl) a...

BACKGROUND Despite the efficacy of the BCR-ABL tyrosine kinase inhibitor Imatinib mesylate for the treatment of chronic myeloid leukemia (CML), resistance has been observed in a proportion of cases, especially those with advanced stages of the disease. Point mutations within the ABL kinase domain are emerging as the most frequent mechanism for reactivation of kinase activity within the leukemic...

The current study describes the short, stability indicative HPLC method development and successive validation of Assay Related Substances methods for Vilazodone Hydrochloride API. This also covers process impurities viz. Impurity A B. chromatographic conditions are similar except sample concentration. proposed utilizes C18 column (15 cm X 4.6 mm, 5 µ, make ZORBAX SB) mobile phase having Methano...

The fluorinated pyrimidine, 5-fluorouracil (FU), has been used in the treatment of a wide variety of solid tumors for more than two decades [l] . It is frequently used in the treatment of early and advanced colorectal carcinomas. Since the drug has a short biological half-life, the clinical pharmacokinetic studies require a sensitive assay procedure which can quantitate low ng/ml FU levels in p...

This study compares cyclosporin A (CsA) concentrations in plasma from patients receiving various transplants, as measured by HPLC and RIA with a monoclonal antibody for CsA and an 125I-labeled ligand. The RIA was restandardized with in-house standards because it overestimated CsA by an average of 23%. The RIA was sensitive to 2 micrograms/L, the standard curve was linear from 20 to 500 microgra...

BACKGROUND The first commercial direct automated immunoassay specific for 25-OH vitamin D(3) (25-OH-D(3)) was recently introduced for use on Roche Diagnostics immunoassay analyzers. We assessed the analytical performance of the Elecsys 25-OH-D(3) assay on a Roche Modular E 170 analyzer. METHODS The Elecsys 25-OH-D(3) assay is a direct electrochemiluminescence immunoassay for human serum or pl...

This study aimed at isolating acylphloroglucinol derivatives with potential antioxidant effect from the brown alga Zonaria tournefortii and to ascertain their structure–activity relationship (SAR). Fractionation HPLC purification led isolation of four major metabolites that were screened for activity via five complementary methods. Results indicated all tested compounds proved have activities. ...