In the moss Physcomitrella patens, transforming DNA containing homologous sequences integrates predominantly by homologous recombination with its genomic target. A systematic investigation of the parameters that determine gene targeting efficiency shows a direct relationship between homology length and targeting frequency for replacement vectors (a selectable marker flanked by homologous DNA). ...

This paper explores the dynamics of evolving cluster stars in presences exotic matter. The $f(R)$ theory is used to presume terms for evolution scenario. We use structure scalars as parameters explore spherically symmetric distribution stars. consider Starobinsky model, $f(R)=R+\epsilon R^2$ and study different modes having features like isotropic pressure, density homogeneity, homologous geode...

DNA repair efficiency is an important determinant of oocyte quality and female fertility. DYNLL1 (Dynein light chain 1) plays a part in double-strand break (DSB) choice by promoting error-prone non-homologous end joining (NHEJ) contrast limiting accurate error-free homologous recombination (HR) repair. Here, we showed that transcript abundance decreases gradually during follicular growth mice. ...

With Machine Translation and/or Automatic Speech Recognition, there can be different versions of the same data with distinct expressions. We argue that combining evidence from these “homologous” datasets can give us better representation of the original data, and our experiments show that a model combining all sources outperforms each individual dataset in retrieval.

Homologous recombination (HR) serves to eliminate deleterious lesions, such as double-stranded breaks and interstrand crosslinks, from chromosomes. HR is also critical for the preservation of replication forks, for telomere maintenance, and chromosome segregation in meiosis I. As such, HR is indispensable for the maintenance of genome integrity and the avoidance of cancers in humans. The HR rea...

The tumor suppressor p53 is a transcription factor whose function is critical for maintaining genomic stability in mammalian cells. In response to DNA damage, p53 initiates a signaling cascade that results in cell cycle arrest, DNA repair or, if the damage is severe, programmed cell death. In addition, p53 interacts with repair proteins involved in homologous recombination. Mitotic homologous r...

The protist Physarum polycephalum is useful for analysis of several aspects of cellular and developmental biology. To expand the opportunities for experimental analysis of this organism, we have developed a method for gene replacement. We transformed Physarum amoebae with plasmid DNA carrying a mutant allele, ardD delta 1, of the ardD actin gene; ardD delta 1 mutates the critical carboxy-termin...

In recent years, the genetic programming crossover operator has been criticized on both theoretical and empirical grounds. This paper introduces a new crossover operator for linear genomes that encourages the emergence of positional homology in the population. Preliminary experimental results suggest that this approach is a promising direction for redesign of the mechanism of crossover.

Many proteoforms - arising from alternative splicing, post-translational modifications (PTMs), or paralogous genes - have distinct biological functions, such as histone PTM proteoforms. However, their quantification by existing bottom-up mass-spectrometry (MS) methods is undermined by peptide-specific biases. To avoid these biases, we developed and implemented a first-principles model (HIquant)...

Homologous recombination can be divided into three key steps: strand exchange, branch migration and resolution. The identification of a protein complex that catalyses branch migration and Holliday junction resolution argues that the mechanism of homologous recombination is conserved from bacteria to man.