The intrinsic features of naive CD4 T cells that affect their ability to respond to polarizing signals for Th cell differentiation are not well understood. In this study, we show that naive CD4 T cells from mice transgenic for the Hlx gene expressed lower levels of IL-4Ralpha. The down-regulation of IL-4Ralpha diminished IL-4 signaling and the Th2 response and enhanced the Th1 response under su...

Criss (Cognitive Psychology 59:297-319, 2009) reported that subjective ratings of memory strength showed a mirror effect pattern in which strengthening the studied words increased ratings for targets and decreased ratings for lures. She interpreted the effect on lure items as evidence for differentiation, a process whereby lures produce a poorer match to strong than to weak memory traces. Howev...

We consider the differentiation of integrals of functions in Besov spaces with respect to the basis of arbitrarily oriented rectangular parallelepipeds in R. We study almost everywhere convergence with respect to Bessel capacities. These outer measures are more sensitive than n-dimensional Lebesgue measure, and therefore we improve the positive results in [4].

Retailers often co-locate spatially to draw consumers, even though it increases price competition. The paper develops a structural model of entry and location choice that isolates the agglomeration benefit of co-location, after controlling for pure differentiation rationales for colocation such as (1) high demand and/or low cost at the location; (2) zoning restrictions and (3) format differenti...

We have analyzed the effect of human recombinant interleukin 4 (rIL-4) on the growth and differentiation of human intrathymic pre-T cells (CD7+2+1-3-4-8-). We describe that this population of T cell precursors proliferates in response to rIL-4 (in the absence of mitogens or other stimulatory signals) in a dose-dependent way. The IL-4-induced proliferation is independent of the IL-2 pathway, as ...

In a paradigm of cellular differentiation, human myoblast fusion, we investigated how a Ca(2+) influx, indispensable for fusion, is triggered. We show how newly expressed Kir2.1 K(+) channels, via their hyperpolarizing effect on the membrane potential, generate a window Ca(2+) current (mediated by alpha 1H T-type Ca(2+) channels), which causes intracellular Ca(2+) to rise.

Despite the great enthusiasm about tissue engineering during the 1980s and the many significant basic observations made since then, the clinical application of tissue-engineered products has been limited. However, the prospect of creating new human tissues and organs is still exciting and continues to be a significant challenge for scientists and clinicians. A human arm is an extremely complica...

Chemical gradient surfaces are described as surfaces with a gradually varying composition along their length. Continuous chemical gradients have recently been proposed as an alternative to discrete microarrays for the high-throughput screening of the effects of ligand concentration in cells. Here, we review some of the most recent examples in which gradients have been used to evaluate the effec...

MicroRNAs are key regulators of the immune response, but their role in CD8 T cell differentiation in vivo is not known. We show that miR-155 is important in both effector and memory antiviral CD8 T cell responses. Without miR-155, there was a weaker effector response and a skewing toward memory precursor cells. At the memory stage, miR-155-deficient CD8 T cells preferentially differentiated int...

Directed differentiation is the process which selectively induce differentiation of stem cells toward definitive cells, but how directed differentiation occurs when stem cells confront the external cues has not been established theoretically. We demonstrate here directed differentiation of a simplified two-component network by using systematic parameter perturbations. These methods and results ...