In this work we provided a selective, sensitive and rapid HPLC-UV method for quantification of sildenafil in human plasma. We have adopted a simple liquid-liquid extraction procedure followed a back-extraction in 5% perchloric acid solution. Chromatographic separation was achieved on a BDS C-18Column (150mm×4.6mm, 5μm) using a mobile phase consisted 63% water, 37% acetonitrile and 0.1% triethyl...

This paper studies the effect of photosensitizer (acetone, H(2)O(2), surfactant and pigment) on photodegradation of diclofenac (DCF) under simulated sunlight. The results demonstrate that degradation pathways proceed via pseudo first-order kinetics in all cases. The photodegradation rate was found to increase with increasing acetone and H(2)O(2). Surfactant and pigment inhibited the photodegrad...

Background and Objectives: A method was developed and validated for determining 15 polycyclic aromatic hydrocarbons (PAHs) in refined olive and refined pomace olive oils using high performance liquid chromatography coupled with a fluorescence detector. Materials and Methods: The sample preparation involved liquid–liquid extraction with organic solvent and low temperature clean-up. The low temp...

The tuned liquid column damper (TLCD) having a uniform cross-sectional tube of U-shaped, occupied with liquid is used as a vibrational response mitigation device. The tuned liquid column ball damper (TLCBD) is a modified TLCD, where, an immovable orifice, positioned at the middle part of the horizontal portion, is replaced by a metal ball. Different studies on the unconstrained optimization per...

results under the above chromatography condition, biuret compound (peqb) and the internal standard were detected at 4.5 and 13.5 minutes, respectively. limit of quantitation of the peqb was 0.1 µg/ml. accuracy of the method over the concentration range of 0.1-100 µg/ml was between 88-109%. inter- and intraday precisions were 4-19% and 6-8%, respectively. a good relationship in the form of a pow...

Poly-L-glutamic acids can exist in random coil, ibility because of its poor pH stability and organic helix, or sheet depending on the environmental conditions. Therefore, they have been used as molecular models for a better understanding of macromolecular stereochemistry [1]. Polyglutamylation is also the major posttranslational modiWcation of neuronal tubulin [2,3], and foliates are polyglutam...