one of the main problems in cell culture is mycoplasma infection. it can extensively affect cell physiology and metabolism. as the applications of cell culture increase in research, industrial production and cell therapy, more concerns about mycoplasma contamination and detection will arise. this review will provide valuable information about: 1. the ways in which cells are contaminated and the...

In the continuous mode of cell culture, a constant flow carrying fresh media replaces culture fluid, cells, nutrients and secreted metabolites. Here we present a model for continuous cell culture coupling intra-cellular metabolism to extracellular variables describing the state of the bioreactor, taking into account the growth capacity of the cell and the impact of toxic byproduct accumulation....

New three-dimensional (3D) scaffolds for bone tissue engineering have been developed throughout which bone cells grow, differentiate, and produce mineralized matrix. In this study, the percentage of cells anchoring to our polymer scaffolds as a function of initial cell seeding density was established; we then investigated bone tissue formation throughout our scaffolds as a function of initial c...

This work attempts to identify growth conditions for maximal productivity of cell mass and of eicosapentaenoic acid (EPA) in ultrahigh cell density cultures of Nannochloropsis sp. Using flat plate reactors with a narrow (1–2 cm) light path and rigorous stirring exposed to high photon flux densities (1000–3000 μmol photons m−# s−"), record population densities (1±2–1±4¬10"! cells ml−") were obta...

background: despite major progress in our general knowledge related to the application of adult stem cells, finding alternative sources for bone marrow mesenchymal stem cells (mscs) has remained to be challenged. in this study successful isolation, multilineage differentiation, and proliferation potentials of sheep mscs derived from bone marrow, adipose tissue, and liver were widely investigate...

The differentiation of murine proadipocytes can be induced by two different methods: 7.) by culture of high-density proad ipocytes in medium containing high concentrations of serum to which is added a variety of hormones and 2.) by culture of lowdensity proadipocytes in heparinized medium containing only human plasma. Phorbol myristate acetate influences differen tiation in both culture systems...

BALB/c mouse embryo cells maintained in tissue culture on a schedule of rapid transfer at high cell density develop into tumorigenic lines that have lost contact inhibition of cell division. After several months in culture, certain of these lines begin to release mouse leukemia virus. This virus has properties indistinguishable from those of virus found in adult BALB/c mice. The evidence presen...