In the present work synthesis of silver nanoparticles using Alyssum homalocarpum (Fisch. & C.A.Mey.) Boiss. extract has been considered. The methanolic extract of A. homalocarpum was concentrated and analyzed using Gas Chromatogra. Also silver nanoparticles were synthesized by the bio-reduction of silver nitrate solution (1 mm) using the methanol extract. Scanning electron microscopy (SEM), and...

Morula (Sclerocarya birrea) beer is a seasonal homemade alcoholic beverage made from morula fruits pulp. Unlike the commercial beverage, alcohol content of not known. The major challenges with beverages arise batch-to-batch differences in product quality and safety due to variability raw materials lack control. Consequently, by same individual may differ. In this study, validated liquid-liquid ...

Volatile organic compounds (VOCs) from uninfested and infested broccoli plant samples with green peach aphid Myzus persicae were investigated by headspace solid microextraction (HS-SPME) combined gas chromatography mass spectrometry (GC-MS). Overall, 25 identified in plants. The HS-SPME GC-MS analysis of the volatiles described differences between samples. Based on peak area analysis, VOCs sign...

Potent antioxidant activities of solvent extracts (96% aqueous ethanol) from the fruit, leaf, and branchlet without adherent leaf Cupressus arizonica were evaluated using 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay compared with butylated hydroxytoluene (BHT) ascorbic acid (AA). Their chemical compositions analyzed gas chromatography-mass spectrometry (GC/MS). Branchlet (BE) most active as an ag...

The volatilomes of both fresh and dried samples of milky mushroom (Calocybe indica P&C var. APK2) were characterized with GCMS/MS. The gas chromatogram was performed with the ethanolic extract of the samples. The results revealed the presence of increased levels of 1, 4:3, 6-Dianhydro-α-dglucopyranose (57.77%) in the fresh and oleic acid (56.58%) in the dried fruiting bodies. The other importan...

A new gene encoding a lipase (designated as Lip-1) was identified from a metagenomic bacterial artificial chromosome(BAC) library prepared from a concentrated water sample collected from a hot spring field in Niujie, Eryuan of Yunnan province in China. The open reading frame of this gene encoded 622 amino acid residues. It was cloned, fused with the oleosin gene and over expressed in Escherichi...

Laminaria japonica L. is among the most commonly consumed seaweeds in northeast Asia. In the present study, L. japonica essential oil (LJEO) was extracted by microwave-hydrodistillation and analyzed by gas chromatography and mass spectroscopy. LJEO contained 21 volatile compounds, comprising 99.76% of the total volume of the essential oil, primarily tetradeconoic acid (51.75%), hexadecanoic aci...

Lipopolysaccharides (LPS) of four nonencapsulated strains of the human enteric pathogen Campylobacter jejuni were chemically characterized. When applied to two of the strains, extraction by a modified phenol-chloroform-petroleum ether method (H. Brade and C. Galanos, Eur. J. Biochem. 122:233-237, 1982) gave better yields of LPS than did extraction by the conventional hot phenol-water technique....

c-AMP dependent protein kinase (protein kinase A, PKA) is an important enzyme involved in the regulation of an increasing number of physiological processes including immune function, cardiovascular disease, memory disorders and cancer. The objective of this study was to evaluate the PKA inhibitory activity of a range of algal extracts, along with their fatty acid composition. Six algal species ...

The taxonomic position of a chlorophenol-degrading bacterium, strain S37T, was investigated. The 16S rDNA sequence indicated that this strain belongs to the genus Sphingopyxis, exhibiting high sequence similarity to the 16S rDNA sequences of Sphingomonas alaskensis LMG 18877T (98.8%), Sphingopyxis macrogoltabida LMG 17324T (98.2%), Sphingopyxis terrae IFO 15098T (95%) and Sphingomonas adhaesiva...