In this study, we analyzed heterosis, amylase enzyme gene expression, and the physiological quality of maize seeds with different genotypes and sizes, which were subjected to aging and not subjected to aging. We used seeds from 2 maize lines that differed with regard to physiological quality, the hybrid, and the reciprocal hybrid; they were classified into 2 sizes and were subjected to aging an...

The therapeutic potential of leaf extract of Bryophyllum pinnatum was screened for in vitro antioxidant potential and alpha amylase inhibitory action. Antioxidant activity of the extract was evaluated for hydroxyl radical scavenging activity by Fenton reaction, free radical scavenging by hydrogen peroxide scavenging and superoxide scavenging potential. The in vitro alpha amylase inhibition acti...

Nitrate induces the formation of nitrate reductase activity in barley (Hordeum vulgare L. cv. Himalaya) aleurone layers. Previous work has demonstrated de novo synthesis of alpha-amylase by gibberellic acid in the same tissue. The increase in nitrate reductase activity is inhibited by cycloheximide and 6-methylpurine, but not by actinomycin D. Nitrate does not induce alpha-amylase synthesis, an...

Shwanniomyces occidentalis possess highly efficient alpha amylase activity that enable this yeast to use raw starch as an carbon source. S.occidentalis alpha amylase gene was cloned into the Saccharomyces cerevisiae under the control of different powerful constitutive promoters, ADH1, GPD and TEF. So that the recombinant strains which carries the same gene under the control of different promote...

Diabetes mellitus has been documented to be associated with alterations in salivary composition and function. Therefore, the main aim of this case-control study was to determine the alterations of salivary alpha-amylase in diabetics compared to healthy people. A total of 40 type 2 diabetic subjects (15 male/ 25 female, aged from 4270 years, mean age 55.6 ±7.11) were selected. Also, the control ...

The subcellular distribution of the enzymes alpha-amylase, protease and ribonuclease in wheat aleurone layers after treatment with gibberellic acid was determined by differential centrifugation. Of the alpha-amylase 56% was precipitable from cell homogenates, indicating that it is a particulate enzyme. Similar results were recorded with protease. Particulate alpha-amylase showed distinct struct...

BACKGROUND Few studies have successfully established an amylase-producing lung cancer cell line or have examined its cytological, biochemical and biological features. PATIENTS AND METHODS Cancer cells, isolated from pleural effusion using a gradient method, were cultivated. RESULTS Amylase production from the newly established cell line was confirmed by positive staining for alpha-amylase a...

In order to characterize the dynamics of the interaction between the emergent membrane translocated exoprotein and the components of Bacillus subtilis cell wall, we examined the kinetics of the in vitro refolding of levansucrase and alpha-amylase, at pH 7 and 37 degrees C, in the presence of polyphosphates (polyP) of various chain lengths (2</=n</=65). These soluble anionic polymers are conside...