the aim of this study was to examine the effect of crude bile on the human hepg2 and ccrf-cem cell lines. cells were exposed to different dilutions of bile. antiproliferative effects were determined by the cytotoxic mtt assay. cells undergoing apoptosis were identified by terminal deoxynucleotidyl transferase dutp nick end labeling (tunel) assay. bile administration resulted in dose-dependent c...

Chromatin disorganization is one of the major alterations linked to prelamin A processing impairment. In this study we demonstrate that BAF is necessary to modulate prelamin A effects on chromatin structure. We show that when prelamin A and BAF cannot properly interact no prelamin A-dependent effects on chromatin occur; similar to what is observed in human Nestor Guillermo Progeria Syndrome cel...

Background and purpose: Cytotoxic effects of Ligustrum vulgare leaves on HeLa cervical tumor cells suggested that extracts should be investigated as potential anticancer agents. Therefore, we examined a antileukemic activity methanolic fruit two types leukemia cells, MOLT-4 JVM-13, lymphocytes isolated from the blood 33 chronic lymphocytic (CLL) patients mononuclear leukocytes 18 healthy indivi...

It is well established that ATP-binding cassette (ABC) transporter-mediated multidrug resistance (MDR) is one of the major mechanisms that causes resistance to antineoplastic drugs in cancer cells. ABC transporters can significantly decrease the intracellular concentration of antineoplastic drugs by increasing their efflux, thereby lowering their cytotoxic activity. One of these transporters, t...

Genome-wide association studies (GWAS) have identified single nucleotide polymorphisms (SNPs) associated with increased risk for colorectal cancer (CRC). A molecular understanding of the functional consequences of this genetic variation is complicated because most GWAS SNPs are located in non-coding regions. We used epigenomic information to identify H3K27Ac peaks in HCT116 colon cancer cells t...

malignant tumors of head and neck carcinomas are the sixth most common type of cancer. current systemic therapies for cancer show side effects in normal tissues and short-term efficacy due to drug resistance. consequently, there is much interest in identifying new drugs for cancer treatment. crocin (an active ingredient of saffron) has been shown to have cytotoxic effects on cancer cell lines. ...

Thimet oligopeptidase (EC 3.4.24.15; EP24.15) was originally described as a neuropeptide-metabolizing enzyme, highly expressed in the brain, kidneys and neuroendocrine tissue. EP24.15 lacks a typical signal peptide sequence for entry into the secretory pathway and is secreted by cells via an unconventional and unknown mechanism. In this study, we identified a novel calcium-dependent interaction...