: This study aimed to evaluate the chemical composition and anticancer activity of Hamadan propolis essential oil methanolic extract on HCT116 cell line. The was evaluated by using MTT (3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide) test colorectal analyzed gas chromatography-flame ionization detection (GC-FID) chromatography-mass spectrometry (GC-MS). total flavonoid phenoli...