Attaching and effacing Escherichia coli (AEEC) strains cause histopathological alterations termed “attaching and effacing (A/E) lesions” (8). The ability to cause A/E lesions is encoded on a large bacterial chromosomal pathogenicity island, the locus of enterocyte effacement (LEE). The central portion of LEE encodes intimin (Eae, 94to 97-kDa outer membrane protein) and Tir, the intimin receptor...

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WITHIN CARDIAC MUSCLE, the myosin heavy chain (MHC) protein is a major component of the contractile machinery. MHC provides structural integrity, and its isoform is a major determinant of contractile and functional properties of the myocardium (1, 8). Cardiac MHC isoforms exist as homologous or heterologous dimers, and a unique gene product encodes each. At the genomic level, and -MHC genes are...

The importance of microenvironment and context in regulation of tissue-specific genes is finally well established. DNA exposure to, or sequestration from, nucleases can be used to detect differences in higher order chromatin structure in intact cells without disturbing cellular or tissue architecture. To investigate the relationship between chromatin organization and tumor phenotype, we utilize...

A LANDMARK STUDY in vascular biology almost 30 years ago described the essential role of the endothelium as a mediator of vasodilator response to acetylcholine (4). Since then many studies have been performed in an effort to understand this complex organ. Endothelial function plays a pivotal role in the maintenance of vascular hemostasis. Endothelial dysfunction is now thought to play an integr...

Couplings between protons, whether scalar or dipolar, provide a wealth of structural information. Unfortunately, the high number of H-H couplings gives rise to complex multiplets and severe overlap in crowded spectra, greatly complicating their measurement. Many different methods exist for disentangling couplings, but none approaches optimum resolution. Here, we present a general new 2D Jresolv...