The present study was first aimed at a complete steady-state kinetic analysis of the reaction between guaiacol (2-methoxyphenol) and the myeloperoxidase (MPO)/H2O2 system, including a description of the isolation and purification of MPO from human polymorphonuclear neutrophil cells. Secondly, the overall reaction of the oxidation of NADPH, mediated by the reactive intermediates formed from the ...

The in vivo and in vitro haemolytic activity of guaiacol glycerol ether has been investigated. Intravenous injections in concentrations of 12.5 per cent or greater produced intravascular haemolysis. The haemolysis produced was not related to the speed of injection. In vitro studies with concentrations of guaiacol glycerol ether confirmed that the critical concentration for the production of hae...

In this study, we have evaluated the co-application of epibrassinolide (24-EBL) and putrescine (Put) on stress indicators and antioxidant system of Solanum lycopersicum L. grown under salinity stress. EBL and Put application along with NaCl stress showed differential responses in the activities of antioxidant enzymes (superoxide dismutase, catalase and guaiacol peroxidase). Significant increase...

Thyroid peroxidase (TPO) was purified from each thyroid of 10 patients (experiment 1) and 4 patients (experiment 2) with toxic diffuse goiter by a simplified method with monoclonal anti-TPO antibody-assisted immunoaffinity column chromatography. The final preparations were used to measure the heme concentration based on the cyanide difference spectrum, and to determine the Km and kcdt values fr...

(Cultural and ligninolytic activity studies of some polypores (Basidiomycota) from brazilian Atlantic Forest, São Paulo State, Brazil). During a fungi survey in the brazilian Atlantic Forest in São Paulo, São Paulo State, Brazil, polypores growing on decaying wood were collected and fragments of the basidiomata were used to obtain isolates in pure culture. A total of 37 isolates were obtained a...

Salicylic acid (SA) is one component of a complex signalling pathway that is induced by a number of biotic and abiotic stresses. Exposing seedling radicles to aqueous solutions of 0.5 mM salicylic acid for 24 h before chilling at 2.5 degrees C for 1-4 days reduced the chilling-induced increase in electrolyte leakage from maize and rice leaves, and cucumber hypocotyls, but not from their radicle...

16 17 The protective effect exerted by polyamines (Put and Spd) against cadmium (Cd) 18 stress was investigated in Brassica juncea plants. Treatment with CdCl2 (75 μM) 19 resulted in a rise of Cd accumulation, a decrease of fresh and dry weights in every 20 plant organ, an increase of free polyamine content at limb and stem levels as well as a 21 decrease at root level. On the other hand, the t...

In this study, the effects of salinity stress (NaCl) on the activity of Catalase (CAT), Guaiacol Peroxidase (POD), and Superoxide dismutase (SOD) were studied in leaves of fifteen oilseed rape (Brassica napus L.) cultivars (Champlain, Hayola401, Licord, Modena, NK Aviator, NK Karibik, NK Octan, Okapy, Opera, PF, RGS, RNX 3621, SLM046, Talayeh, Zarfam). Plants were grown in a greenhouse under co...

A peroxidase (DyP) involved in the decolorization of dyes and produced by the fungus strain Geotrichum candidum Dec 1 was purified. DyP, a glycoprotein, is glycosylated with N-acetylglucosamine and mannose (17%) and has a molecular mass of 60 kDa and an isoelectric point (pI) of 3.8. The absorption spectrum of DyP exhibited a Soret band at 406 nm corresponding to a hemoprotein, and its Na2S2O4-...

A B S T R A C T Evidence for the presence of peroxidative metabolism in rabbit alveolar macrophages (AM) has been obtained from the following observations: (a) catalase is present in high concentrations; (b) peroxidase activity could not be detected employing guaiacol as substrate; (c) the irreversible inhibition of AM catalase by aminotriazole served as a detection system for H202 and demonstr...