recent research highlights the importance of gsts in the establishment of chronic helminth infections. gsts have the potential to protect the parasite against the host immune response. in this present study, gst enzyme assay has been investigated on whole extract of f. hepatica and sheep liver tissue. to the 1-ml plastic cuvette, added 200 mm potassium phosphate buffer. then added 50 mm gsh red...

In mammalian systems, detoxification enzymes of the GST (glutathione S-transferase) family regulate JNK (c-Jun N-terminal kinase) signal transduction by interaction with JNK itself or other proteins upstream in the JNK pathway. In the present study, we have studied GSTs and their interaction with components of the JNK pathway from Diptera. We have evaluated the effects of four Delta class Anoph...

Two cDNA species, aggst1-5 and aggst1-6, comprising the entire coding region of two distinct glutathione S-transferases (GSTs) have been isolated from a 1,1,1-trichloro-2,2-bis-(p-chlorophenyl)ethane (DDT) resistant strain (ZANDS) of Anopheles gambiae. The nucleotide sequences of these cDNA species share 80.2% identity and their derived amino acid sequences are 82.3% similar. They have been cla...

The citrus red mite, Panonychus citri (McGregor), is a global citrus pest, and has developed severe resistance to several types of acaricides. However, the molecular mechanisms of resistance in this mite remain unknown. In this study, seven full-length cDNAs encoding glutathione S-transferases (GSTs) genes were identified and characterized in P. citri. The effects of pyridaben and fenpropathrin...

Glutathione transferases (GSTs) are a superfamily of enzymes which have in common the ability to catalyze nucleophilic addition thiol group reduced glutathione (GSH) onto electrophilic and hydrophobic substrates. This conjugation reaction, occurs spontaneously but is dramatically accelerated by enzyme, protects cells against damages caused harmful molecules. With some exceptions, GSTs catalytic...

The nitroaromatic explosive 2,4,6-trinitrotoluene (TNT) and the related 2,4-dinitrotoluene (DNT) are toxic environmental pollutants. The biotransformation and detoxication of these persistent compounds in higher organisms are of great significance from a health perspective as well as for the biotechnological challenge of bioremediation of contaminated soil. We demonstrate that different human g...