PURPOSE To characterize synthesis and transport of glutathione (GSH) by cultured human retinal pigment epithelial (RPE) cells. METHODS Cultured human RPE cells were depleted of glutathione, then incubated with various concentrations of cysteine, glutamate, and glycine or with glutathione. High-performance liquid chromatography was used to measure intracellular glutathione with time. RESULTS...

To study the role of the redox state regulator glutathione (GSH) in bacterial lipopolysaccharide (LPS)-induced anorexia we measured total reduced GSH (trGSH) in liver, serum and brain in response to intraperitoneal (ip) lipopolysaccharide (LPS, 4 microg/mouse) injection in LPS-naïve and LPS-pretreated (4 microg/mouse given 3 days earlier) mice. LPS reduced food intake in LPS-naïve mice and LPS ...

Leptospirosis is a zoonotic disease that causes severe manifestations such as Weil's disease and pulmonary hemorrhage syndrome. The aim of this study was to evaluate whether reactive oxygen species (ROS) production and antioxidant reduced glutathione (GSH) levels are related to complications in patients hospitalized with leptospirosis. The ROS production and GSH levels were measured in blood sa...

in this research, we investigated the cytotoxic mechanisms of one of the widely used pharmaceuticals that are regularly associated with the adverse effects on the liver, sometimes leading to acute liver failure, diclofenac. diclofenac liver cytotoxicity was associated with reactive oxygen species (ros) formation and lipid peroxidation which were inhibited by antioxidants and ros scavengers, fer...

Taxol is a naturally occurring chemotherapeutic agent that is active against a variety of tumors. Taxol is believed to act by binding tightly to microtubules and preventing their disaggregation. Others have shown that depletion of cellular glutathione results in the disaggregation of microtubules, presumably by allowing the oxidation of some or all of the cysteine residues in tubulins. We studi...

introduction: heavy metals are important occupational and environmental pollutants that cause damage to various organs. although there is no effective therapy for such a poisoning, metallothionein has been shown to play a key role in the detoxification of cadmium (cd). evidence in the literature suggests that superoxide dismutase, glutathione peroxidase, and catalase constitute important defens...

Reduced glutathione (GSH) is a ubiquitous tripeptide found in all eukaryotic cells, including all mammalian tissue. GSH is available to the mammalian organism through cellular synthesis from constituent amino acids, and also through dietary intake. Depletion of GSH in the substantia nigra is one of the earliest reported biochemical events to occur in Parkinson's disease (PD). 1 e GSH content of...

Glutathione (GSH) is one of the most important antioxidants that plays an essential role in detoxification of reactive oxygen species (ROS) which oxidizes to glutathione disulfide (GSSG). Paraquat (PQ), awidely used herbicide, causes pulmonary injury with the productionof ROS. Excessive ROS accumulation as a consequence of PQ exposure are frequently targeted by GSH thereby oxidative stress lead...

Glutathione (GSH) is one of the most important antioxidants that plays an essential role in detoxification of reactive oxygen species (ROS) which oxidizes to glutathione disulfide (GSSG). Paraquat (PQ), awidely used herbicide, causes pulmonary injury with the productionof ROS. Excessive ROS accumulation as a consequence of PQ exposure are frequently targeted by GSH thereby oxidative stress lead...

Background: Acetaminophen (APAP) overdose causes renal and hepatic injury. It is ‎also believed that oxidative stress has a pivotal role in APAP-induced renal injury. ‎Therefore, protective effects of different antioxidants have been examined in APAP-‎induced renal and hepatic toxicity models. Stevia rebadiana is a plant with a high ‎degree of natural antioxidant activity in its leaf extract. T...