The Ca(2+) binding properties of C2 domains are essential for the function of their host proteins. We present here the first crystal structures showing an unexpected Ca(2+) binding mode of the C2B domain of rabphilin-3A in atomic detail. Acidic residues from the linker region between the C2A and C2B domains of rabphilin-3A interact with the Ca(2+)-binding region of the C2B domain. Because of th...

Aim: An ethnomedicinal study was carried out to document the medicinal plants used by ethnic and local villagers around the Veyilingalakona sacred grove located in the eastern ghats of Chittoor District, Andhra Pradesh, India. Methods: Ethnobotanical interviews were carried out on medicinal plants used to treat common ailments based on structural questionnaire with the local people and ethnic (...

The present ethno-botanical exploration conducted in the forest area of Japali Hanuman Theertham a sacred grove of Tirumala hills during the period of 2010-2013 resulted in the information about the uses of traditional medicinal plants of 85 species belonging to 47 families to treat 46 types of ailmentsand utilization of different forest plant resources. Out of 85 plant species the dominant lif...

Expression of the Escherichia coli napFDAGHBC operon (also known as aeg46.5), which encodes the periplasmic molybdoenzyme for nitrate reduction, is increased in response to anaerobiosis and further stimulated by the addition of nitrate or to a lesser extent by nitrite to the cell culture medium. These changes are mediated by the transcription factors Fnr and NarP, respectively. Utilizing a napF...

aromatase inhibitors (ais) as effective candidates have been used in the treatment of hormone-dependent breast cancer. in this study, we have proposed 300 structures as potential ais and filtered them by lipinski’s rule of five using druglito software. subsequently, they were subjected to docking simulation studies to select the top 20 compounds based on their gibbs free energy changes and also...

Sixty taxa (59 species and 1 variety) of lichenized fungi are reported from a chestnut grove in Livari. The majority of them (55 species and 1 variety) occurred on Castanea sativa. The recently described Xylographa soralifera is new to the Balkan Peninsula. The lichenicolous fungus Monodictys epilepraria growing on Lepraria rigidula is new to Montenegro. The lichen mycota is compared with simil...

Determining the mode-of-binding of a DNA ligand is not always straightforward. Here, we establish a scanning force microscopic assay for mode-of-binding that is (i) direct: lengths of individual DNA-ligand complexes are directly measured; (ii) rapid: there are no requirements for staining or elaborate sample preparation; and (iii) unambiguous: an observed increase in DNA length upon addition of...

The catalytic steps of the phospholipase A2 (PLA2)-catalyzed hydrolysis of phospholipids are preceded by interfacial binding. Among various pancreatic PLA2s, bovine pancreatic PLA2 (bpPLA2) has a unique interfacial binding mode in which Lys-56 plays an important role in its binding to anionic lipid surfaces. To identify the structural determinant of this unique interfacial binding mode of bpPLA...

ultrasoundelastographyisanon-invasivemethodwhichimagestheelasticityofsoft-tissues. tomakeanimage, preandafterasmallcompression, ultrasoundradio frequency(rf)signalsareacquiredandthetimedelaysbetweenthemareestimated. thefirstdifferentiationofdisplacementestimationsiscalledelastogram. inthisstudy, wearegoingtomakeanelastogramusingtheprocessingmethodnamed empirical mode decomposition (emd). emdisa...