High-performance liquid chromatography (HPLC), over-pressured-layer chromatography (OPLC) and thin-layer chromatography (TLC) techniques with micellar mobile phases were proposed to evaluate the lipophilicity of 21 newly synthesized 1,2,4-triazoles, compounds of potential importance in medicine or agriculture as fungicides. Micellar parameters log k(m) were compared with extrapolated R(M0) valu...

We propose a reversed phase HPLC (RP-HPLC) with an alkaline-resistant silica-based stationary phase, XBridge Shield RP(18), for the determination of the lipophilicity of drugs with diverse chemical nature ranging from acidic to basic. A set of 40 model compounds with well-defined solvatochromic parameters was selected to allow a broad distribution of structural properties. The chromatographic r...

Application of Sustainable analytical chemistry concepts has become crucial in order to remove the environmentally harmful impacts originating from routine use techniques. Here, a new LC method is developed and its parameters are analyzed, depending on mixed micellar mobile phase. This was primarily aimed at getting rid organic solvents conventional analyses. Combinations tazobactam (TZB) with ...

RP-HPLC METHOD DEVELOPMENT FOR THE QUANTIFICATION OF GABAPENTIN IN FORMULATIONS ABSTRACT A simple, precise and accurate RP-HPLC method was developed and validated for rapid assay of Gabapentin in tablet dosage form. Isocratic elution at a flow rate of 1 mL/min was employed on a symmetry Zodiac C18 (250mmx4.6mm,I.D., 5μm particle size) at ambient temperature. The mobile phase consisted of Methan...

Here, we describe a novel strategy called enzymatic "Mop-Up" that efficiently removes contaminating dNTPs from reverse-phase, high-performance liquid chromatography (RP-HPLC) purified 3'-O-modified dNTP syntheses. Enzymatic mop-up takes advantage of the high selectivity of DNA polymerases for the former nucleoside triphosphates over the latter nucleotide analogs. We demonstrate the selective re...

The regioselective functionalization of four- and six-membered cyclic sulfones was investigated using a lithiation/electrophile trapping strategy. The protocol features an interesting eco-compatibility profile because of the use of 2-MeTHF as a solvent (more eco-friendly than other organic solvents) and n-hexyllithium as a lithiating agent safer than other alkyllithium compounds. Several deriva...

Lamivudine has been widely used in the treatment of HIV disease. A reliable, sensitive reversed phase high performance liquid chromatography (RP-HPLC) method was developed and validated for lamivudine in rabbit plasma. The method was developed on Hypersil BDS C-18 column (250 mm×4.6 mm, 5 μm) using a mobile phase of 0.25% Triethylamine buffer (pH 3.0): acetonitrile (70:30, v/v). The efficient w...

As reported in our previous papers, a series of quinoxaline-2-carboxamide 1,4-di-N-oxide derivatives were synthesized and studied as anti-tuberculosis agents. Here, the capability of the shake-flask method was studied and the retention time (expressed as log K) of 20 compounds were determined by RP-HPLC analysis. We found that the prediction of log P by the RP-HPLC analysis can result in a high...

The present study is carried out to analyze the trace elements sodium, calcium, magnesium, phosphorus, iron, copper, zinc, manganese by atomic absorption spectrometer (AAS) crude protein by Kjeldahl method and essential and non-essential amino acids by reverse phase high performance liquid chromatography (RP-HPLC) from feathers of cock (Gallus domesticus) before the administration of four ACES ...

Objective: To develop a rapid, accurate, linear, sensitive and stability indicating RP-HPLC method for the determination of nilotinib in bulk and pharmaceutical dosage forms in the presence of its four related substances. Methods: The RP-HPLC method was developed for the chromatographic separation of nilotinib and its impurities by using waters Xterra RP-18 (150*4.6 mm, 3.5 μm) column with a mo...