The occurrence of a chloramphenicol-acetylating enzyme, similar to that found in Escherichia coli, carrying an R factor was investigated in various gram-negative bacilli. The acetylated products of chloramphenicol were identified by chromatography and quantitatively assayed after benzene extraction. The investigated strains were of the Salmonella-Arizona group, the Klebsiella-Aerobacter group, ...

Poor effluent management is known to release antibiotic resistance genes and heavy metal into streams. The objective of this study was investigate the occurrence antibiotics metals in hospital effluents streams Benin. extraction genomic DNA from multidrug-resistant bacterial strains isolated stream samples performed according recommendations Quick-DNA TM miniprep kit (Zymo Research Corp, United...

Glucose-nonfermenting gram-negative bacilli (NFB) have been recognized recently as opportunistic pathogens of humans. With few exceptions, strains of NFB have not been considered important enough to be identified when isolated from animals. In this study, all NFB isolated during a 1-year period in a clinical veterinary microbiology laboratory were identified to determine their prevalence. Of th...

This study assessed the recovery rates of Gram-negative bacilli from stored endotracheal aspirates frozen with and without glycerol. Samples frozen with glycerol showed a significant difference in isolate recovery, 89.7% versus 69.2% (P = 0.02). This study demonstrates that it is possible to achieve high recovery rates of potentially pathogenic organisms from endotracheal aspirates when stored ...

The description by Cheron et al. (1) of an outbreak of hospital-acquired infections due toAlcaligenes denitnfi cans and other nonfermenting gram-negative bacilli was reminiscent of a similar outbreak at our hospital. During a 1-month period there were 11 cases of mixed bacterial infections involving Acinetobacter anitratus, Flavobacterium meningosepticum, and Xanthomonas maltophila in our 15-be...

Major problems are being experienced in human medicine from antibiotic resistant bacteria [ 11. Furthermore, no new chemical class of antibiotics has been introduced into medicine in the past two decades [2]. Although many antibiotic structures have been subjected to intensive chemical manipulation to yield novel derivatives with extended activity spectra, resistance to such antibiotics has usu...

A production model of the autoSCAN-4 system (American MicroScan, Inc., Mahwah, N.J.) was tested with not more than 11 strains each of 73 groups or species of gram-negative bacilli from various Centers for Disease Control culture collections. The strains included typical and atypical strains of enteric fermenters, nonenteric fermenters, and nonfermenters. The autoSCAN-4 system identified 95.3% o...

The in-vitro activity of trovafloxacin and nine other antimicrobials was determined for 413 non copy anaerobic clinical isolates. Trovafloxacin was the most active quinolone tested with an MIC90 of 0.5 mg/L against Gram-positive cocci (n = 75); MIC90 of 4 mg/L against Gram-positive bacilli (n = 151); MIC90 of 0.5 mg/L for Gram-negative cocci (n = 12) and MIC90 of 1 mg/L for Gram-negative bacill...